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外文期刊>international journal of immunogenetics
>BIOCHEMICAL ANALYSIS OF A PUBLIC H‐2 SPECIFICITY REVEALED BY AN ANOMALOUS REACTION OF AN ALLOANTISERUM WITH A CHEMICALLY INDUCED C57BL/10 FIBROSARCOMA
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BIOCHEMICAL ANALYSIS OF A PUBLIC H‐2 SPECIFICITY REVEALED BY AN ANOMALOUS REACTION OF AN ALLOANTISERUM WITH A CHEMICALLY INDUCED C57BL/10 FIBROSARCOMA
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机译：BIOCHEMICAL ANALYSIS OF A PUBLIC H‐2 SPECIFICITY REVEALED BY AN ANOMALOUS REACTION OF AN ALLOANTISERUM WITH A CHEMICALLY INDUCED C57BL/10 FIBROSARCOMA
SUMMARYD‐25 is a H‐2 alloantiserum produced in (B10.D2 x C3H.NB) (H‐2dx H‐2p)F1 mice after immunization with B10.RIII(H‐2r) cells, and which is known to recognize the H‐2.25 public antigen onH‐2khaplotypes. The ‘anomalous’ reaction of D‐25 with a partially purified deoxycholate‐solubilized glycoprotein of B10‐1 (H‐2b) fibrosarcoma was studied with various biochemical techniques. The125I‐labelled precipitates were analysed both in one‐ and two‐dimensional SDS‐PAGE and by a partial proteolysis peptide mapping (Cleveland's mapping). The results indicate first that the D‐25‐related antigen was a genuine H‐2 antigen normally associated with the B2‐microglobulin, second that this antigen was borne by the Kbbut not Dbgene products, and finally that D‐25 was able to precipitate the same antigen on normalH‐2bspleen cells. We conclude that this serum recognized a normal H‐2 specificity shared byH‐2bandH‐2rhaplotypes and tentatively identified as the public antigen H‐2.54 which for the first time could be assi
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