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首页> 外文期刊>Acta Physiologiae Plantarum >Metabolic signatures altered by in vitro temperature stress in Ajuga bracteosa Wall. ex. Benth
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Metabolic signatures altered by in vitro temperature stress in Ajuga bracteosa Wall. ex. Benth

机译：Ajuga bracteosa Wall 中体外温度应激改变的代谢特征。前任。本斯

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To elucidate how biosynthesis of plant metabolites is affected by temperature, metabolite profiles from in vitro regenerated plants raised under different temperature regimes of 10, 15 C, 20 C, 25 C and 30 C were obtained using electrospray ionization mass spectrometry (ESI-MS), and principal component analysis (PCA) was carried out to identify key metabolites. Several bin masses were detected by PCA loading scatter plots which separated the samples. In-house bin program selectively manifested the putative known metabolites depending on total ions count and intensity of selected bins in the plant samples. Total phenolic and flavonoid content were harvested to highest levels (12.9 mg GAE/g DW and 9.3 mg QE/g DW), respectively, at 15 C. Besides, pinoresinol (lignan), some of the vital amino acids such as serine, methionine, histidine and glutamine were found to be at higher amount in plants raised at 15 C. Significant phenylpropanoids like cinnamic acid, caffeic acid and quercitol were detected at a higher concentration in plants raised at 15 C as compared to other treatments. However, phosphoenolpyruvate, and oxalosuccinate (intermediates of the pentose phosphate pathway) were accumulated the most in plants raised at 30 C and they were detected with lowest values at 10 C. Glucose and deoxy-xylose 5 phosphate (intermediates of TCA cycle) were found in higher amounts at temperature treatments of 15 and 25 C, respectively. We conclude that a low-temperature treatment (15 C) results in a stress-induced accumulation of a variety of pharmacologically important secondary metabolites.
机译：为阐明温度对植物代谢物生物合成的影响，采用电喷雾电离质谱（ESI-MS）技术获取了在10、15 C、20 C、25 C和30 C不同温度条件下生长的体外再生植物的代谢物谱，并进行了主成分分析（PCA）以鉴定关键代谢物。通过分离样品的 PCA 加载散点图检测了几个箱质量。内部 bin 程序根据植物样品中所选 bin 的总离子计数和强度的百分比选择性地表现出假定的已知代谢物。在15°C下，总酚类和类黄酮含量达到最高水平（分别为12.9 mg GAE/g DW和9.3 mg QE/g DW）。此外，松脂醇（木脂素）、丝氨酸、蛋氨酸、组氨酸和谷氨酰胺等一些重要氨基酸在15°C饲养的植株中含量较高，与其他处理相比，在15°C饲养的植株中检测到肉桂酸、咖啡酸和槲皮醇等苯丙素含量较高。然而，磷酸烯醇丙酮酸和草酰琥珀酸（磷酸戊糖途径的中间体）在30 C饲养的植株中积累最多，在10 C时检测值最低，葡萄糖和脱氧木糖5磷酸（TCA循环中间体）在15°C和25°C的温度处理下含量较高。我们得出的结论是，低温处理（15°C）导致应激诱导的多种药理学上重要的次级代谢物的积累。

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