AbstractToxicity tests revealed up to 40‐fold resistance to a number of cyclodiene insecticides in a laboratory‐reared, cyclodiene‐resistant (CYW) housefly strain (Musca domesticaL.). Using [35S] TBPS as a probe for convulsant sites in insects, saturable specific binding was detected in thorax and abdomen membranes prepared from housefly strains susceptible (CSMA) and resistant (CYW) to cyclodienes. Scatchard analysis of[35S] TBPS binding data to CSMA and CYW membranes failed to provide evidence for significant differences between the two strains in either the affinity (Kd) or density (Bmax) of saturable binding sites. For several polychlorocycloalkane insecticides, the ligand displacement profile of [35S] TBPS binding was almost identical for the CSMA and CYW houseflies. Therefore, using [35S] TBPS as a probe for convulsant sites, a 40‐fold resistance to cyclodienes in the CYW housefly strain cannot be accounted for only in terms of alterations in TBPS bindin
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