首页>
外文期刊>journal of cellular physiology
>Transformation of single myeloid precursor cells by the malignant histiocytosis sarcoma virus (MHSV): Generation of growth‐factor‐independent myeloid colonies and permanent cell lines
【24h】
Transformation of single myeloid precursor cells by the malignant histiocytosis sarcoma virus (MHSV): Generation of growth‐factor‐independent myeloid colonies and permanent cell lines
展开▼
机译：Transformation of single myeloid precursor cells by the malignant histiocytosis sarcoma virus (MHSV): Generation of growth‐factor‐independent myeloid colonies and permanent cell lines
AbstractDirect single‐cell assays for oncogenic transformation are available for fibroblasts but not for other cell types. Using malignant histiocytosis sarcoma virus (MHSV), a member of the ras family of retroviruses, in vivo‐infected granulocyte/macrophage and macrophage precursor cells lost the requirement for externally added hematopoietic growth factors. Factor‐independent growth was demonstrated by colony‐transfer experiments. More than 25% of the independent colonies were established as permanent macrophage cell lines following a phase of adaptation to tissue culture conditions. Factor‐independent colony growth was also obtained by in vitro infection of single cells. As many as 50% of all myeloid precursor cells were target cells for MHSV as measured by this assay. About 2 × 10−3of these colony‐forming cells acquired growth factor independenceandimmortality after in vitro infection. Cell lines derived from these colonies did not require adaptation to tissue cult
展开▼
