AbstractBatch, ammonium‐controlled and simultaneous glucose and ammonium controlled fermentations were compared for the production of protease byBacillus subtilisNCIB 8054. During fermentations ofB. subtilis, maximum protease production was obtained in the stationary phase. Protease production in fermentations controlled at 5 mmol dm−3ammonium was 1.5 times greater than in uncontrolled batch fermentations. Simultaneous control of ammonium at 5 mmol dm−3and glucose at 0.15 g dm−3using controllers based on an ammonium electrode and an oxygen electrode, doubled protease production compared with fermentations having only an ammonium control and tripled protease production compared with uncontrolled batch fermentations. The protease yield on glucose and protease yield on ammonium was increased in fermentations with simultaneous glucose and ammonium
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