...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>journal of cellular physiology >Apotransferrin stimulation of thyroid hormone dependent rat pituitary tumor cell growth in serum‐free chemically defined medium: Role of FE(III) chelation
【24h】

Apotransferrin stimulation of thyroid hormone dependent rat pituitary tumor cell growth in serum‐free chemically defined medium: Role of FE(III) chelation

机译：Apotransferrin stimulation of thyroid hormone dependent rat pituitary tumor cell growth in serum‐free chemically defined medium: Role of FE(III) chelation

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

AbstractTriiodothyronine (T3) dependent growth of GH1rat pituitary tumor cells in serum‐free defined culture requires apotransferrin (apo Tf) (Sirbasku et al.: Mol. Cell. Endocrinol.,77:C47–C55, 1991). Diferric transferrin (2Fe · Tf) also is necessary as an iron source (Eby et al.: Anal. Biochem., 203:317–325, 1992). Further, T3dependence is prevented by soluble Fe(III) addition to the medium (Sato et al.: In Vitro Cell. Dev. Biol., 27A:599–602, 1991). While our data suggested that apo Tf caused growth by chelation of Fe(III), direct evidence was required. We used urea polyacrylamide gel electrophoresis along with autoradiography and Western immunoblotting to measure the Fe(III) content of growing GH1cell cultures and identify the apo Tf, mono‐metal transferrins and 2Fe · Tf present. We found that apo Tf per se did not cause growth but instead chelated inhibitory levels of Fe(III). In fact, apo Tf need not be present at all provided that Fe(III) is reduced to ≤0.6 μM. In addition, other protein and non‐protein Fe(III) chelators were shown to be as effective as apo Tf. Here, we report that pituitary cells are completely inhibited by ≥1.2 μM Fe(III), which are concentrations which might be expected in many culture media and usually are not thought to influence growth. The high sensitivity of pituitary cells to Fe(III) suggests further study to determine what cellular functions are affected and how they interfere with thyroid hormone dependence. ©

著录项

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号