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外文期刊>Arthritis and Rheumatism
>Use of molecular cloning methods to map the distribution of epitopes on topoisomerase I (Scl‐70) recognized by sera of scleroderma patients
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Use of molecular cloning methods to map the distribution of epitopes on topoisomerase I (Scl‐70) recognized by sera of scleroderma patients
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机译：Use of molecular cloning methods to map the distribution of epitopes on topoisomerase I (Scl‐70) recognized by sera of scleroderma patients
AbstractWe report the initial molecular characterization of the autoimmune response against DNA topoisomerase I (topo I; Scl‐70). Sera from 36 patients with scleroderma and 4 healthy control subjects were studied using 6 subcloned portions of topo I. Twenty‐three sera recognized at least 2 independent epitopes on the molecule. Therefore, anti‐topo I, like other non‐organ‐specific autoantibodies characterized to date, is polyclonal and multifocal. The cloned protein should prove suitable for sensitive early detection of anti‐topo I in the clini
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