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>A low molecular weight factor in lung‐conditioned medium stimulating granulocyte and monocyte colony formation in vitro
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A low molecular weight factor in lung‐conditioned medium stimulating granulocyte and monocyte colony formation in vitro
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机译：A low molecular weight factor in lung‐conditioned medium stimulating granulocyte and monocyte colony formation in vitro
AbstractMedium conditioned by excised whole lungs from endotoxin‐injected C57BL mice was highly active in stimulating hemopoietic colony formation, particularly of granulocytic type, in agar cultures of mouse bone marrow cells. The colony stimulating factor (CSF) in this material had an α1‐α2electrophoretic mobility, was eluted from calcium phosphate gel by 0.04Mphosphate buffer and had an unusually low apparent S20Wof 1.9. Sequestered polymor‐phonuclear neutrophils were excluded as a major source of this CSF. The high specific activity and ease of preparation of lung conditioned medium make it valuable both for the large scale production of CSF and as a source of an unusual type
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