Modified Method of Hypoderma Bovis Proteins Transfer Obtained from Gel by Native Electrophoresis onto Nitrocellulose Membrane

TOMASZ CENCEK; JACEK KARAMON; JACEK SROKAJOLANTA ZDYBEL

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Modified Method of Hypoderma Bovis Proteins Transfer Obtained from Gel by Native Electrophoresis onto Nitrocellulose Membrane

机译：Modified Method of Hypoderma Bovis Proteins Transfer Obtained from Gel by Native Electrophoresis onto Nitrocellulose Membrane

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This article presents the essential stages leading to development of a modified method for Hypoderma bovis protein transfer, and estimation of the usefulness of semi-quantitative densitometric analysis of western-blottig results. The principal of the method was to incubate the gel obtained with the native electrophoresis method in buffer containing SDS prior to transfer of the separated proteins of L1 H. bovis onto nitrocellulose membrane. In two experiments, the authors estimated the efficacy of the new method and the possibility of using this method for semi-quantitative densitometric investigations.

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《journal of veterinary research》 |2012年第4期|547-552|共6页
作者
TOMASZ CENCEK; JACEK KARAMON; JACEK SROKAJOLANTA ZDYBEL;
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作者单位

Department of Parasitology and Invasive Diseases, National Veterinary Research Institute;

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原文格式 PDF
正文语种英语
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关键词
Hypoderma bovis; protein; gel transfer; western-blotting; densitometric analysis;

Modified Method of Hypoderma Bovis Proteins Transfer Obtained from Gel by Native Electrophoresis onto Nitrocellulose Membrane

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