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A new method of cloning mammalian tissue culture cells in suspension

机译:A new method of cloning mammalian tissue culture cells in suspension

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AbstractChinese hamster cells (line CHO) were cloned in suspension with a carboxymethylcellulose‐containing medium, and the colonies were counted with an electronic particle counter after enzymatically degrading the carboxymethylcellulose with cellulase. Survival of x‐irradiated cells was determined by both the standard plating technique and the suspension technique, and comparable results were obtai

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