Changes of breath volatile organic compounds in healthy volunteers following segmental and inhalation endotoxin challenge

Holz Olaf; van Vorstenbosch Robert; Guenther FrankSchuchardt SvenTrinkmann Frederikvan Schooten Frederik-JanSmolinska AgnieszkaHohlfeld Jens M

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Changes of breath volatile organic compounds in healthy volunteers following segmental and inhalation endotoxin challenge

机译：Changes of breath volatile organic compounds in healthy volunteers following segmental and inhalation endotoxin challenge

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Abstract It is still unclear how airway inflammation affects the breath volatile organic compounds (VOCs) profile in exhaled air. We therefore analyzed breath following well-defined pulmonary endotoxin (lipopolysaccharide, LPS) challenges. Breath was collected from ten healthy non-smoking subjects at eight time points before and after segmental and whole lung LPS inhalation challenge. Four Tenax-TA® adsorption tubes were simultaneously loaded from an aluminum reservoir cylinder and independently analyzed by two research groups using gas chromatography—mass spectrometry. Airway inflammation was assessed in bronchoalveolar lavage (BAL) and in sputum after segmental and inhaled LPS challenge, respectively. Segmental LPS challenge significantly increased the median (interquartile range, IQR) percentage of neutrophils in BAL from 3.0 (4.2) % to 64.0 (7.3) %. The inhalation challenge increased sputum neutrophils from 33.9 (26.8) % to 78.3 (13.5) %. We observed increases in breath aldehydes at both time points after segmental and inhaled LPS challenge. These results were confirmed by an independent laboratory. The longitudinal breath analysis also revealed distinct VOC patterns related to environmental exposures, clinical procedures, and to metabolic changes after food intake. Changes in breath aldehydes suggest a relationship to LPS induced inflammation compatible with lipid peroxidation processes within the lung. Findings from our longitudinal data highlight the need for future studies to better consider the potential impact of the multiple VOCs from detergents, hygiene or lifestyle products a subject is continuously exposed to. We suspect that this very individual ‘owncloud’ exposure is contributing to an increased variability of breath aldehydes, which might limit a use as inflammatory markers in daily clinical practice.

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《Journal of breath research》 |2022年第3期|共17页
作者
Holz Olaf; van Vorstenbosch Robert; Guenther FrankSchuchardt SvenTrinkmann Frederikvan Schooten Frederik-JanSmolinska AgnieszkaHohlfeld Jens M;
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Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Clinical Airway Research;

Maastricht University;

Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Bio- and Environmental AnalyticsPneumology and Critical Care Medicine, Thoraxklinik at University Hospital Heidelberg, Translational Lung Research Center Heidelberg (TLRC);

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正文语种英语
中图分类内科学;
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airway inflammation; non-invasive monitoring; breath analysis; aldehydes; oxidative stress;

Changes of breath volatile organic compounds in healthy volunteers following segmental and inhalation endotoxin challenge

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