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首页> 外文期刊>journal of cellular physiology >Reorganization of endothelial cord‐like structures on basement membrane complex (Matrigel): Involvement of transforming growth factor β1
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Reorganization of endothelial cord‐like structures on basement membrane complex (Matrigel): Involvement of transforming growth factor β1

机译：Reorganization of endothelial cord‐like structures on basement membrane complex (Matrigel): Involvement of transforming growth factor β1

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AbstractThe formation of capillary‐like network structures by cultured vascular endothelial cells on reconstituted basement membrane matrix, Matrigel, models endothelial cell differentiation, the final step of angiogenesis (Kubota et al., 1988; Grant et al., 1989). When endothelial cells derived from bovine aorta and brain capillaries were plated on Matrigel, DNA synthesis was suppressed and a network of capillary‐like structures rapidly formed in 8–12 h. With time, the network broke down, resulting in dense cellular cords radiating from multiple cellular clusters in 16–24 h. Finally, multicellular aggregates of cells were formed as the network underwent further retraction. Network regression was prevented when either dithiothreitol (DTT) or anti‐TG F‐β1 antibodies were added during the assay. The addition of exogenous TGF‐β1 promoted the regression of endothelial cells into the clusters. This response to TGF‐β1 was blocked by potent serine threonine protein kinase inhibitors, H‐7 and HA 100. TGF‐β1 was released from polymerized Matrigel by incubation with Dulbecco's modified eagle's medium (DMEM) in the absence of cells. The Matrigel‐conditioned DMEM inhibited endothelial DNA synthesis even in the presence of anti‐TGF‐β1 antibodies. These results suggest that TGF‐β1 and possibly other soluble factors from Matrigel may be important for differentitation and remodeling of endothelial cells in a capillary network with possible implications for wound healing and development. © 1994 Wiley‐Liss, Inc.This article is a US Government work and as such, is in the public do

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