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首页> 外文期刊>The Journal of toxicological sciences >Avian eggshell thinning caused by transovarian exposure to o,p '-DDT: changes in histology and calcium-binding protein production in the oviduct uterus
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Avian eggshell thinning caused by transovarian exposure to o,p '-DDT: changes in histology and calcium-binding protein production in the oviduct uterus

机译：经卵巢暴露于o，p'-DDT引起的禽蛋壳变薄：输卵管子宫内组织学和钙结合蛋白产生的变化

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Reproductive disorders in birds are the most characteristic effects of DDT contamination of wildlife. Experimental exposure of avian eggs to the estrogenic substance o,p'-DDT causes abnormal development of the reproductive tract (shortening of the left oviduct and aberrant development of the right oviduct) and eggshell thinning in mature birds, but it is still not known how eggshell thinning occurs in the abnormal oviduct. To fill this information gap, we examined the histology of the uterine part of the oviduct in Japanese quail treated in ovo with o,p'-DDT or a synthetic estrogen, diethylstilbestrol (DES), and we performed immunohistochemical staining for the calcium-binding proteins CALB1, SPP1, and TRPV6. Both o,p'-DDT-treated and DES-treated quail had few, and scattered, gland cells in the left uterus, unlike vehicle controls, in which gland cells tightly occupied the lamina propria. The aberrantly developed right uterus retained all the components of the normal left uterus, but in immature form. Immunostaining for CALB1, SPP1, and TRPV6 was greatly reduced by both o,p'-DDT and DES; SPP1 and TRPV6 immunostaining patterns, in particular, differed distinctly from those in the controls. These findings suggest that CALB1, SPP1, and TRPV6 are molecular factors, decreased production of which is responsible for eggshell thinning. Our findings also could contribute to understanding of the eggshell formation mechanism in birds.
机译：鸟类的生殖障碍是滴滴涕污染野生动物的最典型影响。禽蛋实验性地暴露于雌激素物质o，p'-DDT会导致成年鸟类生殖道发育异常（左输卵管缩短和右输卵管发育异常）和蛋壳变薄，但目前还不知道异常输卵管中蛋壳变薄是如何发生的。为了填补这一信息空白，我们检查了用 o，p'-DDT 或合成雌激素己烯雌酚 （DES） 卵子处理的日本鹌鹑输卵管子宫部分的组织学，并对钙结合蛋白 CALB1、SPP1 和 TRPV6 进行了免疫组织化学染色。o，p'-DDT处理和DES处理的鹌鹑在左子宫中都很少且分散的腺体细胞，这与载体对照不同，其中腺体细胞紧紧占据固有层。发育异常的右子宫保留了正常左子宫的所有成分，但形式不成熟。o，p'-DDT 和 DES 大大降低了 CALB1、SPP1 和 TRPV6 的免疫染色;特别是SPP1和TRPV6免疫染色模式与对照组明显不同。这些发现表明，CALB1、SPP1 和 TRPV6 是分子因子，其产量减少是导致蛋壳变薄的原因。我们的研究结果也有助于理解鸟类蛋壳的形成机制。

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