EFFECT OF EFFLUX PUMP INHIBITORS ON ANTIMICROBIAL ACTIVITY OF FLORFENICOL AGAINST RESISTANT STRAINS OF PORCINE ACTINOBACILLUS PLEUROPNEUMONIAE AND PASTEURELLA MULTOCIDA

Porjai Rattanapanadda; Hung-Chih Kuo; Ming-Kun Hsieh

首页> 外文期刊>Taiwan Veterinary Journal >EFFECT OF EFFLUX PUMP INHIBITORS ON ANTIMICROBIAL ACTIVITY OF FLORFENICOL AGAINST RESISTANT STRAINS OF PORCINE ACTINOBACILLUS PLEUROPNEUMONIAE AND PASTEURELLA MULTOCIDA

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EFFECT OF EFFLUX PUMP INHIBITORS ON ANTIMICROBIAL ACTIVITY OF FLORFENICOL AGAINST RESISTANT STRAINS OF PORCINE ACTINOBACILLUS PLEUROPNEUMONIAE AND PASTEURELLA MULTOCIDA

机译：EFFECT OF EFFLUX PUMP INHIBITORS ON ANTIMICROBIAL ACTIVITY OF FLORFENICOL AGAINST RESISTANT STRAINS OF PORCINE ACTINOBACILLUS PLEUROPNEUMONIAE AND PASTEURELLA MULTOCIDA

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The aim of this study is to investigate whether combinational use of efflux pump inhibitors (EPIs) could improve florfenicol (FF) antimicrobial activity. Five EPIs including Carbonyl Cyanide Chlorophenylhydrazone (CCCP), omeprazole, Phenylalanine-arginine beta-naphthylamide (PAbetaN), reserpine and verapamil were individually combined with FF and the minimum inhibitory concentration (MIC) against porcine Actinobacillus pleuropneumoniae and Pasteurella multocida were evaluated by the broth microdilution abetaay. The results indicated that CCCP demonstrated substantial improvement on the antimicrobial activity (FF MIC reduction ≥4 folds) while PAbetaN showed minimal effect at high concentrations (80–120mug/mL). The MICs of FF were further examined with CCCP at 5mug/mL against resistant A. pleuropneumoniae and at 2mug/mL against resistant P. multocida. With this combination, a total of 75% (6/8) of A. pleuropneumoniae and 100% (8/8) of P. multocida resistant isolates showed significantly reduced (≥4 folds) FF MICs. In addition, 100% of the 16 resistant bacterial strains carried the floR gene that regulates the phenicol-specific efflux pump (FloR pump). The time kill experiments were used to verify the effect of CCCP demonstrating a bactericidal effect in resistant A. pleuropneumoniae and synergistic effect in resistant P. multocida. In contrast, the FF MICs were not significantly affected in the FF susceptible strains of these two bacteria. These findings suggested that despite small sample sizes, consistent beneficial effects of CCCP were evident such that the combinational use of selective EPI with FF might be an alternative antibacterial strategy against FF resistant A. pleuropneumoniae and P. multocida. The anti-efflux mechanism was indicated, but more researches to further decipher the mechanisms of CCCP’s effects are warranted.

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《Taiwan Veterinary Journal》 |2020年第3期|151-160|共10页
作者
Porjai Rattanapanadda; Hung-Chih Kuo; Ming-Kun Hsieh;
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作者单位

Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 145 XingDa Road, Taichung, Taiwan;

Department of Veterinary Medicine, College of Veterinary Medicine, National Chiayi University, 580 Xinmin Road, Chiayi, Taiwan;

Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, 145 XingDa Road, Taichung, Taiwan;

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正文语种英语
中图分类畜牧、动物医学、狩猎、蚕、蜂;
关键词
Efflux pump inhibitors (EPIs); Florfenicol (FF); A. pleuropneumoniae; P. multocida and Carbonyl Cyanide Chlorophenylhydrazone;

EFFECT OF EFFLUX PUMP INHIBITORS ON ANTIMICROBIAL ACTIVITY OF FLORFENICOL AGAINST RESISTANT STRAINS OF PORCINE ACTINOBACILLUS PLEUROPNEUMONIAE AND PASTEURELLA MULTOCIDA

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