A solid phase radioimmunoassay (SP-RIA) was developed for the determination of urinary conjugated aldosterone. Acid-hydrolyzed urine was extracted with dichloromethane and the extract was analyzed by SP-RIA. Microtiter plates were coated with two different anti-aldosterone antisera (S-3: against aldosterone-21-hemisuccinate-BSA and 58: aldosterone-3-(-0-carboxymethyl)-oxime-BSA separately and preincubated with3H-aldosterone. Reference values were obtained using a liquid radioimmunoassay method including paper chromatography. Regression analysis revealed for S-3: Y = 0.8 X + 3.2, r = 0.84; p 0.0001; n = 36 and for 58: Y = 1.16 X + 1.2, r = 0.89; p 0.0001; n = 36.
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