...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and biosystems engineering >Studies on production and evaluation of biopigment and synthetic dye decolorization capacity of laccase produced by A. oryzae cultivated on agro-waste
【24h】

Studies on production and evaluation of biopigment and synthetic dye decolorization capacity of laccase produced by A. oryzae cultivated on agro-waste

机译：Studies on production and evaluation of biopigment and synthetic dye decolorization capacity of laccase produced by A. oryzae cultivated on agro-waste

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

The present study investigated the screening of mono and co-culture fungal cultivations for laccase production using extracted lignin as the substrate obtained from cauliflower wastes by two different pretreatment methods. Amongst mono and mixed culture fungal cultivations, monoculture of Aspergillus oryzae exhibited the highest enzymatic activity of 29.7 +/- 0.6 U mL(-1) under submerged conditions and using alkali extracted lignin as substrate. Under the optimal conditions (pH 4.5, 30 degrees C, 12 days, 1% (w/v) lignin and 0.5 mM Cu2+ concentration) the maximum laccase activity was estimated to be 41.3 +/- 2.8 U mL(-1) and production yield of 153.3 +/- 2.4 mg L-1. Maximum decolorization of pigment extracted from Aspergillus heteromorphus CBS 117.55 cultivated culture media was achieved by administration of 40 U g(-1) of crude enzyme concentration. Thermal and pH stability of crude laccase was observed over wide ranges. The dye decolorization efficiency of crude A. oryzae laccase was studied and Congo Red exhibited maximum decolorization percentage (64 +/- 1.3%) at 15 mu M, 50 degrees C and pH 4.5. The kinetic study of different dye (Congo Red) concentrations obtained V-max and K-m values of 0.123 x 10(-3) M and 0.724 mol L-1 min(-1), respectively.

著录项

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号