CitWRKY28 and CitNAC029 promote the synthesis of cuticular wax by activating CitKCS gene expression in citrus fruit

Yang Hongbin; Zhu Zhifeng; Zhang MingfeiLi XinXu RangweiZhu FengXu JuanDeng XiuxinCheng Yunjiang

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CitWRKY28 and CitNAC029 promote the synthesis of cuticular wax by activating CitKCS gene expression in citrus fruit

机译：CitWRKY28 and CitNAC029 promote the synthesis of cuticular wax by activating CitKCS gene expression in citrus fruit

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Cuticular wax covers the fruit surface, playing important roles in reduction of fruit water loss and resistance to pathogen invasion. However, there is limited research on the synthesis and transcriptional regulation of cuticular wax in citrus fruit. In this study, we characterized the variations of aliphatic wax in HJ (Citrus reticulata) and ZK (Citrus trifoliata) from young fruit to mature fruit, as well as performed transcriptome sequencing on 27 samples at different fruit developmental stages. The results revealed that the ZK fruit always had a higher aliphatic wax content than the HJ fruit during development. qRT-PCR analysis demonstrated that two KCS genes, CitKCS1 and CitKCS12, had the most significant difference in expression between HJ and ZK. Furthermore, a heterologous expression assay in Arabidopsis indicated that CitKCS1 and CitKCS12 are involved in cuticular wax synthesis. Subsequently, gene co-expression network analysis screened CitWRKY28 and CitNAC029. Dual luciferase and EMSA assays indicated that CitWRKY28 might bind to the promoter of CitKCS1 and CitKCS12 and CitNAC029 might bind to that of CitKCS1 to activate their expression. Moreover, CitWRKY28 and CitNAC029 could promote the accumulation of cuticular wax in Arabidopsis leaves. Our findings provide new insights into the synthesis and regulation of cuticular wax and valuable information for further mining of wax-related genes in citrus fruit.

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来源
《Plant Cell Reports》 |2022年第4期|905-920|共16页
作者
Yang Hongbin; Zhu Zhifeng; Zhang MingfeiLi XinXu RangweiZhu FengXu JuanDeng XiuxinCheng Yunjiang;
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Natl R&D Ctr Citrus Postharvest Technol;

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正文语种英语
中图分类植物细胞学;
关键词
Citrus fruit; Cuticular wax; WRKY; NAC; beta-Ketoacyl-CoA synthase (KCS); TRANSPIRATION BARRIER PROPERTIES; TRANSCRIPTION FACTOR; ARABIDOPSIS; CHAIN; ACID; BIOSYNTHESIS; SINENSIS; ENCODES; RESISTANCE; TOLERANCE;

CitWRKY28 and CitNAC029 promote the synthesis of cuticular wax by activating CitKCS gene expression in citrus fruit

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