Mouse–mouse somatic hybridization techniques were used to produce monoclonal antibodies directed towards antigen expressed on retinoblastoma–derived tissue culture cell lines. Four monoclonal antibodies were produced with varying avidity which were reactive against allogeneic retinoblastoma–associated antigens. Sensitivity and specificity was demonstrated using ELISA and chromium release assays. Immunofluorescent antibody studies suggest that these monoclonal antibodies may be useful diagnostically.
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