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>Transformation of mouse bone marrow cells by transfection with a human oncogene related to c‐myc is associated with the endogenous production of macrophage colony stimulating factor 1
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Transformation of mouse bone marrow cells by transfection with a human oncogene related to c‐myc is associated with the endogenous production of macrophage colony stimulating factor 1
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机译：Transformation of mouse bone marrow cells by transfection with a human oncogene related to c‐myc is associated with the endogenous production of macrophage colony stimulating factor 1
AbstractWe recently derived a series of transformed cell lines by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with a newly described human gene, R‐myc, which has homology to the c‐myc oncogene. In this report, we show that these lines share some features characteristic of cells of the mononuclear phagocyte lineage. Specifically, all cell lines had macrophage‐or monocytelike morphology, contained nonspecific esterase, were phagocytic for latex beads, secreted lysozyme, bore the Mac‐1 antigen, and contained a minority of cells with Fc receptors. However, only a single monocytelike clone had appreciable numbers of cells which bore complement receptor 1, and none were phagocytic for antibody or complementcoated particles, or constitutively secreted Interleukin‐1. All these cell lines secreted a growth factor capable of supporting the in vitro proliferation of bone marrow macrophages. Radiommunoassay and receptor binding studies indicate that this factor is colony stimulating
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