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首页> 外文期刊>journal of cellular physiology >Effects of recombinant human granulocyte‐macrophage colony‐stimulating factor (GM‐CSFrh) on transmembrane electrical potentials in granulocytes: Relationship between enhancement of ligand‐mediated depolarization and augmentation of superoxide anion (O −2) production
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Effects of recombinant human granulocyte‐macrophage colony‐stimulating factor (GM‐CSFrh) on transmembrane electrical potentials in granulocytes: Relationship between enhancement of ligand‐mediated depolarization and augmentation of superoxide anion (O −2) production

机译：Effects of recombinant human granulocyte‐macrophage colony‐stimulating factor (GM‐CSFrh) on transmembrane electrical potentials in granulocytes: Relationship between enhancement of ligand‐mediated depolarization and augmentation of superoxide anion (O −2) production

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AbstractWhen human granulocytes that have been primed with recombinant human granulocyte–macrophage colony‐stimulating factor (GM‐CSFrh) are activated by ligands that stimulate the respiratory burst, the amount of superoxide anion (O−2) they generate is significantly increased. We have found that the accelerated rate of O−2release occurring under these conditions is accompanied by an antecedent increase in membrane depolarization. We examined the nature of the enhancement of membrane depolarization in GM‐CSFrh‐primed granulocytes and investigated its relationship to the increase in O −2generation by N‐formyl methionylleucylphenylalanine (fMLP)‐activated granulocytes. We found that augmented depolarization could not be accounted for by a change in the resting membrane potential induced by the growth factor and was still present after either blocking passive transmembrane Na+movement with dimethylamiloride or by increasing the membrane's permeability to K+with valinornycin. When their ability to depolarize was virtually eliminated by dissipating the transmembrane K+gradient, GM‐CSFrh‐pretreated cells continued to generate more O −2after fMLP than did control cells. These results indicate that augmentation of the granulocyte's ability to generate O −2anions, which is induced by priming with GM‐CSFrh, is independent both of the resting transmembrane potential and of alterations in the extent of membrane potential cha

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