Porous Alginate Scaffolds Designed by Calcium Carbonate Leaching Technique

Wulf Alena; Mendgaziev Rais I; Fakhrullin RawilVinokurov VladimirVolodkin DmitryVikulina Anna S.

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Porous Alginate Scaffolds Designed by Calcium Carbonate Leaching Technique

机译：Porous Alginate Scaffolds Designed by Calcium Carbonate Leaching Technique

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One of the main challenges in modern tissue engineering is to design biocompatible scaffolds with finely tuned porous architecture and capacity to load bioactive molecules that guide the growth and differentiation of the cells during tissue reconstruction. This work proposes a strategy to design porous alginate scaffolds (PAS) with well-tuned architecture by leaching of sacrificial vaterite CaCO3 microspheres packed in alginate. Pore size and interconnectivity depend on CaCO3 sphere dimensions and packing as well as alginate concentration. Varying of these parameters, almost hundred percent pore interconnectivity (or, by contrast, a zero pore interconnectivity) can be achieved. Junctions between interconnected pores are about 50-70% of the pore dimensions that provides molecular transport through the PASs potentially ensuring diffusion of nutrition, oxygen and metabolic products when cell seeding. An opportunity to fabricate a multifunctional scaffold is demonstrated by encapsulation of desired macromolecules into the individual pores of a scaffold (is illustrated by dextran loading). Mechanical properties of PASs are found typical for soft and hydrated structures (Young's modulus of 19 +/- 15 kPa) which is appropriate for cell seeding. The three cell lines (HeLa, HEK293, and L929) are cultured on different alginate scaffolds to examine cell viability and adhesiveness.

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《Advanced functional materials》 |2022年第14期|2109824.1-2109824.10|共10页
作者
Wulf Alena; Mendgaziev Rais I; Fakhrullin RawilVinokurov VladimirVolodkin DmitryVikulina Anna S.;
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Friedrich Alexander Univ Erlangen Nurnberg FAU, Bavarian Polymer Inst, Dr Mack Str 77, D-90762 Furth, Germany;

Fraunhofer Inst Cell Therapy & Immunol, Fraunhofer IZI BB, Branch Bioanalyt & Bioproc, Muhlenberg 13, D-14476 Potsdam, Germany;

Gubkin Russian State Univ Oil & Gas, Dept Phys Chem, Leninsky Pr 65-1, Moscow 119991, RussiaGubkin Russian State Univ Oil & Gas, Dept Phys Chem, Leninsky Pr 65-1, Moscow 119991, Russia|Kazan Fed Univ, Inst Fundamental Med & Biol, Kreml Urami 18, Kazan 420008, Republic Of Tat, RussiaGubkin Russian State Univ Oil & Gas, Dept Phys Chem, Leninsky Pr 65-1, Moscow 119991, Russia|Nottingham Trent Univ, Sch Sci & Technol, Clifton Lane, Nottingham NG11 8NS, England;

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正文语种英语
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CaCO; (3); cell culture; encapsulation; hydrogel; porosity;

Porous Alginate Scaffolds Designed by Calcium Carbonate Leaching Technique

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