Region specificity of fibroblast-like cells in the mucosa of the rat large intestine

Tamura Saki; Mantani Youhei; Nakanishi SatokiOhno NobuhikoYokoyama ToshifumiHoshi Nobuhiko

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Region specificity of fibroblast-like cells in the mucosa of the rat large intestine

机译：Region specificity of fibroblast-like cells in the mucosa of the rat large intestine

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Our previous studies using immunohistochemistry and serial block-face scanning electron microscopy (SBF-SEM) clarified that fibroblast-like cells (FBLCs) in the rat ileal mucosa are classifiable into several subtypes, but their characteristics throughout the large intestine remain unknown. In this study, we investigated the region-specific characteristics of FBLCs in the rat large intestine using histological analysis including SBF-SEM. Immunohistochemistry revealed that CD34(+)CD31(-) FBLCs were localized in the lamina propria beneath the crypt bases throughout the large intestine and were more abundant in the descending colon than in the other regions. In addition, platelet-derived growth factor receptor alpha (PDGFR alpha)(+) FBLCs were ubiquitously present just below the epithelium throughout the large intestine, and those at the crypt base were slightly more abundant in the descending colon than in the other regions. SBF-SEM analysis revealed that there were two types of FBLCs around the crypt base in both the cecum and the descending colon: sub-epithelial FBLCs localizing just beneath the epithelium in the manner of PDGFR alpha(+) FBLCs, and lamina propria FBLCs localizing farther away from the epithelium than sub-epithelial FBLCs in the manner of CD34(+)CD31(-) FBLCs. The lamina propria FBLCs were closely apposed to various immune cells in the lamina propria, and their endoplasmic reticulum in the descending colon exhibited various dilatation levels, unlike that in the cecum. These findings indicate that FBLCs, especially around the crypt base, differed in each region of the large intestine with respect to localization, abundance, and ultrastructure, which could lead to the region-specific microenvironment around the crypt base.

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来源
《Cell and Tissue Research》 |2022年第3期|427-441|共15页
作者
Tamura Saki; Mantani Youhei; Nakanishi SatokiOhno NobuhikoYokoyama ToshifumiHoshi Nobuhiko;
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作者单位

Lab Anim Mol Morphol,Kobe Univ;

Kobe University,Kobe Univ;

Sch Med,Jichi Med Univ;

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正文语种英语
中图分类细胞生物学;
关键词
Fibroblast-like cell; Immunofluorescent analysis; Large intestine; Rat; Serial block-face scanning electron microscopy; ENDOPLASMIC-RETICULUM STRESS; LAMINA PROPRIA; REPLICATION MIGRATION; COLONIC-MUCOSA; PRIMARY CILIUM; NORMAL RABBIT; PANETH CELLS; ADULT TISSUE; SYSTEM; CYTODIFFERENTIATION;

Region specificity of fibroblast-like cells in the mucosa of the rat large intestine

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