Distribution and quantification of activated platelets in platelet-rich fibrin matrices

Sato Atsushi; Kawabata Hideo; Aizawa HachidaiTsujino TetsuhiroIsobe KazushigeWatanabe TaisukeKitamura YutakaMiron Richard J.Kawase Tomoyuki

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Distribution and quantification of activated platelets in platelet-rich fibrin matrices

机译：Distribution and quantification of activated platelets in platelet-rich fibrin matrices

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Platelet-rich fibrin (PRF) has been widely applied in regenerative therapy owing to its simple preparation protocol. To date, the original protocol for preparing leukocyte-rich (L)-PRF has been modified to produce derivatives such as advanced (A)-PRF, concentrated growth factors (CGF), and horizontal (H)-PRF. However, these derivatives have not been rigorously compared to explore possible differences. We previously developed and validated a nondestructive near-infrared (NIR) imaging method to quantitatively examine the platelet distribution in PRF matrices. To further evaluate the characteristics of platelets in PRF, we herein examined the distribution of activated platelets. Four types of PRF matrices were prepared under different centrifugal conditions from blood samples obtained from the same healthy donors. After fixation and compression, the matrices were stained immunohistochemically without sectioning and visualized using an NIR imager. Qualitative morphological analysis revealed that whole platelets were distributed widely and homogeneously in H-PRF and A-PRF, but localized along the distal tube surface in L-PRF and CGF. Activated platelets were distributed as were whole platelets in A-PRF, L-PRF, and CGF, but localized mainly in the "buffy coat" region in H-PRF. Quantitative analysis revealed that there was no significant difference in the ratio of activated to whole platelets between PRF derivatives. These findings suggest that platelet activation is similarly induced in fibrin matrices regardless of centrifugal speed or rotor angulation. However, only the H-PRF group was distinguishable from the other PRF derivatives in terms of activated platelet distribution.

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来源
《Platelets》 |2022年第1期|110-115|共6页
作者
Sato Atsushi; Kawabata Hideo; Aizawa HachidaiTsujino TetsuhiroIsobe KazushigeWatanabe TaisukeKitamura YutakaMiron Richard J.Kawase Tomoyuki;
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作者单位

Dent Hosp,Nihon Univ;

Collaborat Res Grp,Tokyo Plast Dent Soc;

Inst Med & Dent,Niigata UnivDiv Oral & Maxillofacial Surg,Matsumoto Dent Univ HospDept Periodontol,Univ Bern;

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正文语种英语
中图分类基础医学;
关键词
Activated platelets; CD41; CD62P; distribution; platelet-rich fibrin;

Distribution and quantification of activated platelets in platelet-rich fibrin matrices

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