Fish eDNA detection and its technical optimization: A case study of Acanthopagrus latus

Sun Meijing; Guo Yu; Zhao NaiqianZhang ShuoPei KunQin Chuanxin

首页> 外文期刊>Marine environmental research >Fish eDNA detection and its technical optimization: A case study of Acanthopagrus latus

【24h】

Fish eDNA detection and its technical optimization: A case study of Acanthopagrus latus

机译：Fish eDNA detection and its technical optimization: A case study of Acanthopagrus latus

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

The development of a standardized eDNA detection process is the primary step in improving the accuracy and efficiency of eDNA detection. In this study, primers and probes with high specificity were selected to identify the COI gene of Acanthopagrus latus. Through experiments on the influence of different water quantities, methods of water sample preservation and water bathing times on the result of eDNA detection, the accuracy of this method for extracted water samples was improved. Specifically, a water bathing time of 6 h provided an optimal eDNA concentration from the water sample. After 6 h, the concentration began to decrease, so 6 h was determined to be the best water bathing time for A. latus. Five water extraction volumes (250 mL, 500 mL, 1 L, 2 L, and 3 L) were tested, and there was a positive correlation between water extraction volume and the DNA concentration in the water sample. Different water sample preservation methods were also compared, and it was found that at = 7 d, the concentration obtained with the cryopreservation method for different water extraction volumes was higher than that obtained with the ethanol preservation method. In this study, we established and optimized a technical procedure for eDNA-based detection of A. latus in aquatic environments. We hope to apply this method in field investigations and provide a reference for the study of eDNA in other fishes.

著录项

来源
《Marine environmental research》 |2022年第4期|105588.1-105588.9|共9页
作者
Sun Meijing; Guo Yu; Zhao NaiqianZhang ShuoPei KunQin Chuanxin;
展开▼
作者单位

Shanghai Ocean Univ, Coll Marine Sci, Shanghai 201306, Peoples R China|Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China;

Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China|Natl Agr Expt Stn Fishery Resources & Environm Da, Shenzhen 518120, Peoples R China;

Fangchenggang Fishery Radio, Fangchenggang 538001, Peoples R ChinaShanghai Ocean Univ, Coll Marine Sci, Shanghai 201306, Peoples R ChinaFangchenggang Fisheries Technol Extens Stn, Fangchenggang 538001, Peoples R China;

展开▼
收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类
关键词
Environmental DNA; Detection technology; Water filter; Preservation method; Water bathing time;

Fish eDNA detection and its technical optimization: A case study of Acanthopagrus latus

摘要

著录项

相关主题

期刊订阅