Subcellular distribution of alpha 2-adrenoceptor subtypes in the rodent kidney

Shimokawa Takaomi; Nakagawa Toshitaka; Hayashi KoheiYamagata MasayoYoneda Kozo

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Subcellular distribution of alpha 2-adrenoceptor subtypes in the rodent kidney

机译：Subcellular distribution of alpha 2-adrenoceptor subtypes in the rodent kidney

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Renal alpha 2-adrenoceptors have been reported to play a role in the regulation of urinary output, renin secretion, and water and sodium excretion in the kidneys. However, the distribution of alpha 2-adrenoceptor subtypes in the kidneys remains unclear. In this study, we aimed to investigate the localization of alpha 2-adrenoceptor subtypes in rat kidneys using 8-week-old Sprague-Dawley rats. Immunofluorescence imaging revealed that both alpha 2A- and alpha 2B-adrenoceptors were expressed in the basolateral, but not apical, membrane of the epithelial cells of the proximal tubules. We also found that alpha 2A- and alpha 2B-adrenoceptors were not expressed in the glomeruli, collecting ducts, or the descending limb of the loop of Henle and vasa recta. In contrast, alpha 2C-adrenoceptors were found to be localized in the glomeruli and lumen of the cortical and medullary collecting ducts. These results suggest that noradrenaline may act on the basement membrane of the proximal tubules through alpha 2A- and alpha 2B-adrenoceptors. Moreover, noradrenaline may be involved in the regulation of glomerular filtration and proteinuria through the induction of morphological changes in mesangial cells and podocytes via alpha 2C-adrenoceptors. In the collecting ducts, urinary noradrenaline may regulate morphological changes of the microvilli through alpha 2C-adrenoceptors. Our findings provide an immunohistochemical basis for understanding the cellular targets of alpha 2-adrenergic regulation in the kidneys. This may be used to devise therapeutic strategies targeting alpha 2-adrenoceptors.

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《Cell and Tissue Research》 |2022年第2期|303-314|共12页
作者
Shimokawa Takaomi; Nakagawa Toshitaka; Hayashi KoheiYamagata MasayoYoneda Kozo;
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作者单位

Fac Pharm,Osaka Ohtani Univ;

Life Sci Res Ctr,Kagawa Univ;

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正文语种英语
中图分类细胞生物学;
关键词
Kidney; alpha 2-adrenoceptor; Distribution; Catecholamine; Immunohistochemistry; ADRENERGIC-RECEPTOR SUBTYPES; VACUOLAR H+-ATPASE; VASOACTIVE SUBSTANCES; MESANGIAL CELLS; MESSENGER-RNA; V-ATPASE; RAT; ALPHA(2)-ADRENOCEPTOR; EXPRESSION; INJURY;

Subcellular distribution of alpha 2-adrenoceptor subtypes in the rodent kidney

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