Elevated microRNA-7 inhibits proliferation and tumor angiogenesis and promotes apoptosis of gastric cancer cells via repression of Raf-1

Lin Jing; Liu Zewa; Liao ShashaLi E.Wu XiaohuaZeng Wanting

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Elevated microRNA-7 inhibits proliferation and tumor angiogenesis and promotes apoptosis of gastric cancer cells via repression of Raf-1

机译：Elevated microRNA-7 inhibits proliferation and tumor angiogenesis and promotes apoptosis of gastric cancer cells via repression of Raf-1

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Objective Since the essential involvement of microRNAs (miRNAs) in the development and progression of GC, the study was for the exploration of the value of microRNA-7 (miR-7) in the evaluation of neoadjuvant chemotherapy for gastric cancer (GC) and its effects on apoptosis, proliferation and angiogenesis of GC. Methods miR-7 expression in serum of GC patients before and after neoadjuvant chemotherapy were detected to explore its role in neoadjuvant chemotherapy of GC. The GC cells were transfected with miR-7 mimics/inhibitors, or siRNA-Raf-1 to figure out their roles in proliferation, migration, invasion, cycle distribution and apoptosis. Tumor xenograft was conducted to test tumor growth. Microvessel density (MVD) in tumors was tested by immunohistochemical staining. Results miR-7 expression in serum of GC patients was lower than that of healthy controls while it was elevated after neoadjuvant chemotherapy. Moreover, higher miR-7 expression was exhibited in chemotherapy-effective patients rather than chemotherapy-ineffective patients (P< 0.01). miR-7 expression in serum was connected with tumor size, degree of differentiation, TNM stage and lymphatic metastasis.miR-7 was decreased and Raf-1 was elevated in GC cells (bothP< 0.05). Elevated miR-7 or declined Raf-1 inhibited GC cell migration, proliferation and invasion, cell cycle entry, xenografted tumor growth and MVD and stimulated apoptosis (allP< 0.05). Down-regulated Raf-1 reversed the impacts of miR-7 knockdown on GC cells (allP< 0.05). Conclusion Our study highlights that elevated miR-27a indicates the good efficacy of neoadjuvant chemotherapy in GC and miR-7 targets Raf-1 to suppress tumor development and angiogenesis of GC cells.

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《Cell cycle》 |2020年第19期|2496-2508|共13页
作者
Lin Jing; Liu Zewa; Liao ShashaLi E.Wu XiaohuaZeng Wanting;
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作者单位

Shantou Univ, Oncol Dept, Coll Med, Affiliated Hosp 1, Shantou, Peoples R China;

Shantou Longhu Peoples Hosp, Oncol Dept, Shantou, Guangdong, Peoples R China;

UCL, MSci Appl Med Sci, Div Med, London WC1E 6BT, England;

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正文语种英语
中图分类细胞生物学;
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MicroRNA-7; gastric cancer; neoadjuvant chemotherapy; Raf-1; proliferation; migration; invasion; apoptosis;

Elevated microRNA-7 inhibits proliferation and tumor angiogenesis and promotes apoptosis of gastric cancer cells via repression of Raf-1

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