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首页> 外文期刊>Acta Physiologiae Plantarum >Different roles of inducible and constitutive HSP70 and HSP90 in tolerance of Arabidopsis thaliana to high temperature and water deficit
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Different roles of inducible and constitutive HSP70 and HSP90 in tolerance of Arabidopsis thaliana to high temperature and water deficit

机译：诱导型和组成型HSP70和HSP90在拟南芥耐高温和缺水性中的不同作用

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摘要

Considering global warming, it is of critical interest to understand in detail how plants withstand elevated temperatures and drought. Heat shock proteins (HSP) are involved in cellular response to both factors. HSP70 and HSP90 are multigene families in plants that function as molecular chaperones throughout the organism's tolerance range. However, the roles of individual members under water deficit are much less understood compared to heat shock response. In this study, transcriptional profiling of 12 HSP70s and 7 HSP90s in Arabidopsis thaliana (L.) Heynh. seedlings within wide dose ranges of high temperature and dehydration was conducted by RT-PCR, and roles of distinct inducible and constitutive members in tolerance were evaluated using loss-of-function mutants. The cellular responses to both treatments included strong factor-specific induction of inducible members and mild up-regulation of most constitutive members of various subcellular compartments. Differences in their kinetics, including baseline, time course and magnitude of changes, indicate specific functions of individual members. A significant complement to the cytosolic HSP transcript pool was created through differential expression of three inducible genes: AtHSP70-4 was highly induced in both cases, whereas induction of AtHSP70-5 and AtHSP90-1 was strong at heat shock and barely detectable or absent during dehydration. Nevertheless, analysis of knockout plants showed that AtHSP70-5 and AtHSP90-1 not only significantly contributed to the seed and seedling thermotolerance, but also substantially supported the growth activity under water deficit, when their expression remained negligible. These results suggested that inducible HSP70 and HSP90 at different levels of their expression perform specialized functions in protecting cells under adverse conditions.
机译：考虑到全球变暖，详细了解植物如何承受高温和干旱至关重要。热休克蛋白 （HSP） 参与细胞对这两种因素的反应。HSP70 和 HSP90 是植物中的多基因家族，在整个生物体的耐受范围内充当分子伴侣。然而，与热休克反应相比，个体成员在缺水作用下的作用知之甚少。在这项研究中，拟南芥 （L.） Heynh 中 12 个 HSP70 和 7 个 HSP90 的转录谱分析。采用RT-PCR技术对高温脱水宽剂量范围内的幼苗进行研究，并利用功能丧失突变体评估不同诱导型和组成型成员在耐受性中的作用。对两种处理的细胞反应包括诱导型成员的强因子特异性诱导和各种亚细胞区室的大多数组成成员的轻度上调。其动力学的差异，包括基线、时程和变化幅度，表明了各个成员的特定功能。通过三个诱导基因的差异表达，对胞质 HSP 转录库产生了显着的补充：AtHSP70-4 在两种情况下都高度诱导，而 AtHSP70-5 和 AtHSP90-1 的诱导在热休克时很强，在脱水期间几乎检测不到或不存在。然而，对基因敲除植株的分析表明，AtHSP70-5和AtHSP90-1不仅对种子和幼苗的耐热性有显著贡献，而且在缺水的情况下也显著支持了生长活性，而它们的表达量可以忽略不计。这些结果表明，不同表达水平的诱导型 HSP70 和 HSP90 在不利条件下在保护细胞方面发挥特殊功能。

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