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首页> 外文期刊>Russian journal of marine biology >A Study of Polyphenol Fractions in an Extract from the Brown Alga Thalassiophyllum clathrus (Postels Ruprecht, 1840) and Their Antioxidant Activities
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A Study of Polyphenol Fractions in an Extract from the Brown Alga Thalassiophyllum clathrus (Postels Ruprecht, 1840) and Their Antioxidant Activities

机译：A Study of Polyphenol Fractions in an Extract from the Brown Alga Thalassiophyllum clathrus (Postels Ruprecht, 1840) and Their Antioxidant Activities

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A number of polyphenol fractions were extracted from the brown alga Thalassiophyllum clathrus(Postels & Ruprecht, 1840) by the method of liquid–liquid extraction using solvents of increasing polarity,and their antioxidant activities were studied. The extract obtained with ethyl acetate was characterized by amaximum polyphenol content of 581.2 ± 8.4 mg/g equivalent to gallic acid and a maximum antioxidant activity(22.0 ± 0.1 μg/mL in EC50 units (2,2-diphenyl-1-picrylhydrazyl) and 5.84 ± 1.64 μmol/g equivalent toTrolox). Six purified polyphenol fractions were isolated from the ethyl acetate extract by preparative sizeexclusionchromatography; the antioxidant activity was preserved. The conditions for separation of the purifiedpolyphenol fractions through reverse phase chromatography were selected as follows: the pH of elutionbuffer was 2.0–3.0; the water–acetonitrile gradient was supplemented with formic acid; the temperature was25℃; and the flow rate was 1 mL/min. It has been shown that the extract from T. clathrus contains polyphenoliccompounds of the following groups: phenolic acids (gallic acid), hydroxycinnamic acids (caffeic acidand its derivative, chlorogenic acid), and flavonols (kaempferol and quercetin).

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