Cross-linked enzyme aggregates (CLEAs) of cellulase with improved catalytic activity, adaptability and reusability

Li Tiantian; Gong Xiaowu; Yang GaoshanLi QinHuang JinZhou NaJia Xin

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Cross-linked enzyme aggregates (CLEAs) of cellulase with improved catalytic activity, adaptability and reusability

机译：Cross-linked enzyme aggregates (CLEAs) of cellulase with improved catalytic activity, adaptability and reusability

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In this study, cross-linked cellulase aggregates (C-CLEAs) were synthesized by precipitation of cellulase with ammonium sulfate and then cross-linking with glutaraldehyde. The results revealed that the optimal pH of C-CLEAs shifted toward a more acidic environment by 2.0 pH units, and the optimal temperature shifted toward higher temperature by 20 degrees C after immobilization. The half-life (t(1/2)) and inactivation energy (E-d) values of the C-CLEAs were 5.98 times and 1.93 times than that of free cellulase, respectively. Moreover, the C-CLEAs can also maintain about 65.22% of activity after 10 cycles and 63.03% of activity after storage for 56 days at 4 degrees C. Enzymatic hydrolysis of carboxymethylcellulose sodium and corncob in C-CLEAs system verified that the C-CLEAs performed better than free cellulase (P 0.01).

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来源
《Bioprocess and biosystems engineering》 |2022年第5期|865-875|共11页
作者
Li Tiantian; Gong Xiaowu; Yang GaoshanLi QinHuang JinZhou NaJia Xin;
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作者单位

Shihezi Univ, Key Lab Green Proc Chem Engn Xinjiang Bingtuan, Sch Chem & Chem Engn, Beisi Rd, Xinjiang 800032, Peoples R China;

Southwest Univ, Sch Chem & Chem Engn, Chongqing, Peoples R China|Southwest Univ, Chongqing Key Lab Soft Matter Mat Chem Funct Mfg, Chongqing, Peoples R China|Southwest Univ, Belt & Rd B&R Int Joint Res Lab Sustainable Mat, Chongqing, Peoples R China;

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正文语种英语
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Catalytic activity; Cross-linked cellulase aggregates; Enzymatic saccharification; Thermal stability;

Cross-linked enzyme aggregates (CLEAs) of cellulase with improved catalytic activity, adaptability and reusability

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