Using Culture-Enriched Phenotypic Metagenomics for Targeted High-Throughput Monitoring of the Clinically Important Fraction of the β-Lactam Resistome

Zhiguo Zhang; Guoqing Zhang; Feng Ju

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Using Culture-Enriched Phenotypic Metagenomics for Targeted High-Throughput Monitoring of the Clinically Important Fraction of the β-Lactam Resistome

机译：Using Culture-Enriched Phenotypic Metagenomics for Targeted High-Throughput Monitoring of the Clinically Important Fraction of the β-Lactam Resistome

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摘要

High bacterial community diversity and complexity greatly challenge the cost-efficient monitoring of clinically prevalent antibiotic-resistant bacteria,which are usually present as rare and important populations involved in the environmental dissemination of clinical resistance.Here,we introduce culture-enriched phenotypic metagenomics that integrates culture enrichment,phenotypic screening,and metagenomic analyses as an emerging standardized methodology for targeted resistome monitoring and apply it to decipher the extended-spectrum β-lactam resistome in a municipal wastewater treatment plant e(WWTP)and its receiving river.The results showed that clinically prevalent carbapenemase genes(e.g.,the NDM and KPC families)and extended-spectrum β-lactamase genes(e.g.,the CTX-M,TEM,and OXA families)were prevalent in the WWTP and showed prominent potential in horizontal dissemination.Strikingly,carbapenem and polymyxin resistance genes co-occurred in the highly virulent nosocomial pathogens Enterobacter kobei and Citrobacter freundii.Overall,this study exemplifies phenotypic metagenomics for high-throughput surveillance of a targeted clinically important fraction of antibiotic resistomes and substantially expands current knowledge on extended-spectrum β-lactam resistance in WWTPs.

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《Environmental Science & Technology: ES&T》 |2022年第16期|11429-11439|共11页
作者
Zhiguo Zhang; Guoqing Zhang; Feng Ju;
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作者单位

Key Laboratory of Coastal Environment and Resources of Zhejiang Province,School of Engineering,Westlake University,Hangzhou 310030 Zhejiang China;

Institute of Advanced Technology,Westlake Institute for Advanced Study,Hangzhou 310024 Zhejiang China;

Key Laboratory of Coastal Environment and Resources of Zhejiang Province,School of Engineering Westlake University,Hangzhou 310030 Zhejiang China;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类环境科学、安全科学;
关键词
phenotypic metagenomics; carbapenemases; extended-spectrum β-lactamases(ESBLs); wastewater treatment plants; microbiome;

Using Culture-Enriched Phenotypic Metagenomics for Targeted High-Throughput Monitoring of the Clinically Important Fraction of the β-Lactam Resistome

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