Analysis of the Persistence and Particle Size Distributional Shift of Sperm-Derived Environmental DNA to Monitor Jack Mackerel Spawning Activity

Satsuki Tsuji; Hiroaki Murakami; Reiji Masuda

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Analysis of the Persistence and Particle Size Distributional Shift of Sperm-Derived Environmental DNA to Monitor Jack Mackerel Spawning Activity

机译：Analysis of the Persistence and Particle Size Distributional Shift of Sperm-Derived Environmental DNA to Monitor Jack Mackerel Spawning Activity

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Environmental DNA(eDNA)analysis holds great promise as an efficient and noninvasive method to monitor not only the distribution of organisms but also their spawning activity.In eDNA analysis-based monitoring of spawning activity,the detection of sperm-derived eDNA is a key point; however,its characteristics and dynamics are completely unknown.The present study focuses on the persistence and particle size distribution(PSD)of eDNA derived from the sperm of Japanese jack mackerel.First,we investigated the time-dependent degradation and the PSD of sperm-derived eDNA by artificially adding sperm to seawater.Next,we kept fish in tanks and examined the changes in eDNA concentration and PSD before and after spawning.The results of two experiments showed that the degradation of sperm-derived eDNA proceeded rapidly,with PSD shifting to a smaller size regardless of the DNA region(Cyt b or ITS1).Additionally,it was shown that the nuclei and mitochondria released from sperm through degradation had a size distribution that was not simply dependent on each organelle size.These results will contribute to elucidating the characteristics and dynamics of eDNA specifically during the spawning season and to further developing eDNA analysis as a powerful tool for the monitoring of spawning activity.

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《Environmental Science & Technology: ES&T》 |2022年第15期|10754-10763|共10页
作者
Satsuki Tsuji; Hiroaki Murakami; Reiji Masuda;
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作者单位

Center for the Promotion of Interdisciplinary Education and Research,Kyoto University,Kyoto 606-8SO2,Japan;

Maizuru Fisheries Research Station,Field Science Education and Research Center,Kyoto University,;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类环境科学、安全科学;
关键词
spawning activity; sperm-derived eDNA; particle size distribution(PSD); degradation; Japanese jack mackerel;

Analysis of the Persistence and Particle Size Distributional Shift of Sperm-Derived Environmental DNA to Monitor Jack Mackerel Spawning Activity

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