Ectopic expression of nucleolar DEAD-Box RNA helicaseOsTOGR1confers improved heat stress tolerance in transgenic Chinese cabbage

Yarra Rajesh; Xue Yongbiao

首页> 外文期刊>Plant Cell Reports >Ectopic expression of nucleolar DEAD-Box RNA helicaseOsTOGR1confers improved heat stress tolerance in transgenic Chinese cabbage

【24h】

Ectopic expression of nucleolar DEAD-Box RNA helicaseOsTOGR1confers improved heat stress tolerance in transgenic Chinese cabbage

机译：Ectopic expression of nucleolar DEAD-Box RNA helicaseOsTOGR1confers improved heat stress tolerance in transgenic Chinese cabbage

获取原文

获取原文并翻译 | 示例

获取外文期刊封面封底 >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Key message The DEAD-Box RNA helicase OsTOGR1positively regulates heat stress tolerance in Chinese cabbage. Non-heading Chinese cabbage (Brassica rapaL. ssp.chinensis) is primarily cultivated vegetable crop in Asian countries. Heat stress is one of the major threats for its growth and yield. Numerous regulatory genes in various crops have shown to contribute thermotolerance. Among them, Thermotolerant growth required 1 (TOGR1) is an important DEAD-box RNA helicase. To examine whether its role is conserved in other crops, we constructedpCAMBIA1300-pHSP:OsTOGR1expression vector driven by the rice small heat shock protein promoter (pHSP17.9) and successfully produced transgenic non-heading Chinese cabbage plants expressingOsTOGR1gene viaAgrobacterium-mediated vacuum infiltration transformation.In total, we generated three independent transgenic cabbage lines expressingTOGR1gene. Expression and integration ofTOGR1was confirmed by PCR, RT-PCR and qPCR in T(1)and T(2)generations. The relative leaf electrical conductivity of transgenic seedlings was reduced subjected to high temperature (38 degrees C) compared to heat shock treatment (46 degrees C). In addition, hypocotyl length of transgenic seedlings increased compared to wild-type plants under high temperature and heat shock treatment. Furthermore, the transgenic plants exhibited higher chlorophyll content than wild-type plants under high temperature and heat shock treatment. The transgenic seeds displayed better germination under heat shock treatment. Tested heat stress-responsive genes were also up-regulated in the transgenic plants subjected to high temperature or heat shock treatment. To the best of our knowledge, this is the first report on describing the role of DAED-Box RNA helicases in improving heat stress tolerance of transgenic plants.

著录项

来源
《Plant Cell Reports》 |2020年第12期|1803-1814|共12页
作者
Yarra Rajesh; Xue Yongbiao;
展开▼
作者单位

Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类植物细胞学;
关键词
TOGR1; thermotolerant growth required 1; HSP; heat shock protein; Non-heading Chinese cabbage;

Ectopic expression of nucleolar DEAD-Box RNA helicaseOsTOGR1confers improved heat stress tolerance in transgenic Chinese cabbage

摘要

著录项

相关主题

期刊订阅