Screening of Endocrine Disrupting Potential of Surface Waters via an Affinity-Based Biosensor in a Rural Community in the Yellow River Basin,China

Jisui Tan; Lanhua Liu; Fangxu Li

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Screening of Endocrine Disrupting Potential of Surface Waters via an Affinity-Based Biosensor in a Rural Community in the Yellow River Basin,China

机译：Screening of Endocrine Disrupting Potential of Surface Waters via an Affinity-Based Biosensor in a Rural Community in the Yellow River Basin,China

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Overcoming the limitations of traditional analytical methods and developing technologies to continuously monitor environments and produce a comprehensive picture of potential endocrine-disrupting chemicals(EDCs)has been an ongoing challenge.Herein,we developed a portable nuclear receptor(NR)-based biosensor within 90 min to perform highly sensitive analyses of a broad range of EDCs in environmental water samples.Based on the specific binding of the fluorescence-labeled NRs with their ligands,the receptors were attached to the EDC-functionalized fiber surface by competing with EDCs in the samples.The biosensor emitted fluorescence due to the evanescent wave excitation,thereby resulting in a turn-off sensing mode.The biosensor showed a detection limit of 5 ng/L E2-binding activity equivalent(E2-BAE)and 93 ng/L T3-BAE.As a case study,the biosensor was used to map the estrogenic binding activities of surface waters obtained from a rural community in the Yellow River basin in China.When the results obtained were compared with those from the traditional yeast two-hybrid bioassay,a high correlation was observed.It is anticipated that the good universality and versatility exhibited by this biosensor for various EDCs,which is achieved by using different NRs,will significantly promote the continuous assessment of global EDCs.

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《Environmental Science & Technology: ES&T》 |2022年第20期|14350-14360|共11页
作者
Jisui Tan; Lanhua Liu; Fangxu Li;
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作者单位

State Key Joint Laboratory of ESPC,School of Environment,Tsinghua University,Beijing 100084,China;

School of Ecology and Environmental Science,Zhengzhou University,Zhengzhou 4S0001,China;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类环境科学、安全科学;
关键词
endocrine-disrupting chemicals; indirect competition bioassay; nuclear receptors; evanescent wave biosensor; affinity-based; rural community;

Screening of Endocrine Disrupting Potential of Surface Waters via an Affinity-Based Biosensor in a Rural Community in the Yellow River Basin,China

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