Circular RNA circSLC8A1 inhibits the proliferation and invasion of non-small cell lung cancer cells through targeting the miR-106b-5p /FOXJ3 axis

Yong Wenmu; Deng Shujiao; Tan YunfangLi Sen

首页> 外文期刊>Cell cycle >Circular RNA circSLC8A1 inhibits the proliferation and invasion of non-small cell lung cancer cells through targeting the miR-106b-5p /FOXJ3 axis

【24h】

Circular RNA circSLC8A1 inhibits the proliferation and invasion of non-small cell lung cancer cells through targeting the miR-106b-5p /FOXJ3 axis

机译：Circular RNA circSLC8A1 inhibits the proliferation and invasion of non-small cell lung cancer cells through targeting the miR-106b-5p /FOXJ3 axis

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Circular RNA derived from the SLC8A1 gene (circSLC8A1) has been implicated in the pathogenesis of several types of cancers. However, the role of circSLC8A1 in non-small cell lung cancer (NSCLC) remains unclear. In the present study, the expression levels of circSLC8A1 in NSCLC tissues and cell lines were determined by qRT-PCR analysis. Function-gain-assays were then carried out to further validate the role of circSLC8A1 in NSCLC in vitro. Online prediction software and the subsequent luciferase reporter assay were used to identify the target genes of circSLC8A1 and microRNA (miR)-106b-5p. CircSLC8A1 was found to be downregulated in NSCLC tissues and cell lines. Overexpression of circSLC8A1 significantly inhibited the proliferation and invasion of NSCLC cells. Further investigations shown that circSLC8A1 was able to bind to miR-106b-5p as well as inhibit the expression of miR-106b-5p in NSCLC cells. MiR-106b-5p mimics reversed the inhibitory effects of circSLC8A1 overexpression on cell proliferation and invasion. Furthermore, we found that forkhead box J3 (FOXJ3) to be a target gene of miR-106b-5p in NSCLC cells. Knockdown of FOXJ3 reversed the inhibitory effects of miR-106b-5p inhibitor on cell proliferation and invasion. Collectively, these findings indicate that circSLC8A1 exhibits anti-tumor activity in NSCLC, which might be mediated by the miR-106b-5p/FOXJ3 axis. The circSLC8A1/miR-106b-5p/FOXJ3 axis may thus represent a promising therapeutic target for the management of NSCLC.

著录项

来源
《Cell cycle》 |2021年第24期|2597-2606|共10页
作者
Yong Wenmu; Deng Shujiao; Tan YunfangLi Sen;
展开▼
作者单位

Dept Resp & Crit Care Med,HanZhong Cent Hosp;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
non-small cell lung cancer; miR-106b-5p; FOXJ3; invasion; PROGRESSION;

Circular RNA circSLC8A1 inhibits the proliferation and invasion of non-small cell lung cancer cells through targeting the miR-106b-5p /FOXJ3 axis

摘要

著录项

相关主题

期刊订阅