Blue Light Switchable Cell–Cell Interactions Provide Reversible and Spatiotemporal Control Towards Bottom‐Up Tissue Engineering

Yüz Simge G.; Rasoulinejad Samaneh; Mueller MarcWegner Anatol E.Wegner Seraphine V.

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Blue Light Switchable Cell–Cell Interactions Provide Reversible and Spatiotemporal Control Towards Bottom‐Up Tissue Engineering

机译：Blue Light Switchable Cell–Cell Interactions Provide Reversible and Spatiotemporal Control Towards Bottom‐Up Tissue Engineering

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Abstract Controlling cell–cell interactions is central for understanding key cellular processes and bottom‐up tissue assembly from single cells. The challenge is to control cell–cell interactions dynamically and reversibly with high spatiotemporal precision noninvasively and sustainably. In this study, cell–cell interactions are controlled with visible light using an optogenetic approach by expressing the blue light switchable proteins CRY2 or CIBN on the surfaces of cells. CRY2 and CIBN expressing cells form specific heterophilic interactions under blue light providing precise control in space and time. Further, these interactions are reversible in the dark and can be repeatedly and dynamically switched on and off. Unlike previous approaches, these genetically encoded proteins allow for long‐term expression of the interaction domains and respond to nontoxic low intensity blue light. In addition, these interactions are suitable to assemble cells into 3D multicellular architectures. Overall, this approach captures the dynamic and reversible nature of cell–cell interactions and controls them noninvasively and sustainably both in space and time. This provides a new way of studying cell–cell interactions and assembling cellular building blocks into tissues with unmatched flexibility.

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《Advanced Biosystems》 |2019年第4期|n/a-n/a|共7页
作者
Yüz Simge G.; Rasoulinejad Samaneh; Mueller MarcWegner Anatol E.Wegner Seraphine V.;
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作者单位

Max Planck Institute for Polymer ResearchAckermannweg 10,55128,Mainz,Germany;

Department of Statistical ScienceUniversity College LondonGower Street,London,WC1E 6BT,UK;

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原文格式 PDF
正文语种英语
中图分类生物科学;
关键词
bottom‐up tissue engineering; cell adhesion; cell–cell interactions; photoswitchable proteins; spatiotemporal control;

Blue Light Switchable Cell–Cell Interactions Provide Reversible and Spatiotemporal Control Towards Bottom‐Up Tissue Engineering

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