Head on Comparison of Self- and Nano-Assemblies of Gamma Peptide Nucleic Acid Amphiphiles

Malik Shipra; Kumar Vikas; Liu Chung-HaoShih Kuo-ChihKrueger SusanNieh Mu-PingBahal Raman

首页> 外文期刊>Advanced functional materials >Head on Comparison of Self- and Nano-Assemblies of Gamma Peptide Nucleic Acid Amphiphiles

【24h】

Head on Comparison of Self- and Nano-Assemblies of Gamma Peptide Nucleic Acid Amphiphiles

机译：Head on Comparison of Self- and Nano-Assemblies of Gamma Peptide Nucleic Acid Amphiphiles

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Peptide nucleic acids (PNAs) are nucleic acid analogs with hybridization properties and enzymatic stability superior to that of DNA. In addition to gene targeting applications, PNAs have garnered significant attention as bio-polymers due to the Watson-Crick-based molecular recognition and flexibility of synthesis. Here, PNA amphiphiles are engineered using chemically modified gamma PNA (8 mer in length) containing hydrophilic diethylene glycol units at the gamma position and covalently conjugated lauric acid (C12) as a hydrophobic moiety. Gamma PNA (gamma PNA) amphiphiles self-assemble into spherical vesicles. Further, nano-assemblies (NA) are formulated using the amphiphilic gamma PNA as a polymer via ethanol injection-based protocols. Comprehensive head-on comparison of the physicochemical and cellular uptake properties of PNA derived self- and NA is performed. Small-angle neutron and X-ray scattering analysis reveal ellipsoidal morphology of gamma PNA NA that results in superior cellular delivery compate to the spherical self-assembly. Next, the functional activities of gamma PNA self-and NA in lymphoma cells via multiple endpoints, including gene expression, cell viability, and apoptosis-based assays are compared. Overall, it is established that gamma PNA amphiphile is a functionally active bio-polymer to formulate NA for a wide range of biomedical applications.

著录项

来源
《Advanced functional materials》 |2022年第7期|2109552.1-2109552.12|共12页
作者
Malik Shipra; Kumar Vikas; Liu Chung-HaoShih Kuo-ChihKrueger SusanNieh Mu-PingBahal Raman;
展开▼
作者单位

Univ Connecticut, Dept Pharmaceut Sci, Storrs, CT 06269 USA;

Univ Connecticut, Inst Mat Sci, Polymer Program, 191 Auditorium Rd, Storrs, CT 06269 USA;

NIST, Gaithersburg, MD 20899 USAUniv Connecticut, Inst Mat Sci, Polymer Program, 191 Auditorium Rd, Storrs, CT 06269 USA|Univ Connecticut, Dept Chem & Biomol Engn, Storrs, CT 06269 USA|Univ Connecticut, Dept Biomed Engn, Storrs, CT 06269 USA;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类
关键词
gamma peptide nucleic acids; micro-RNA-155; nano-assemblies; peptide nucleic acid amphiphiles; self-assembly;

Head on Comparison of Self- and Nano-Assemblies of Gamma Peptide Nucleic Acid Amphiphiles

摘要

著录项

相关主题

期刊订阅