A Bio-Functional Wnt3a Gradient Microarray for Cell Niche Studies

Xinna Wang; Wanjing Ou; Di FengBo GaoBarbara Pui Chan

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A Bio-Functional Wnt3a Gradient Microarray for Cell Niche Studies

机译：A Bio-Functional Wnt3a Gradient Microarray for Cell Niche Studies

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The Wingless/Integrated (Wnt) signaling is a central player in regulating multiplecellular events ranging from developmental process, adult homeostasisto tumorigenesis. In native microenvironments, Wnt signals often functionthrough the concentration gradients between the secreting site and the targetingsite. Hence, presenting Wnt molecules in a concentration gradient invitro in a controllable manner recapitulates the native Wnt niche. Nevertheless,most in vitro Wnt niche models, where the ligands are homogeneously immobilizedon material surface, fail in reconstituting the concentration gradient ofWnt as that in vivo. Herein, using a previously established Multiphoton Microfabricationand Micropatterning (MMM) technology, a concentration gradientof Wnt3a is immobilized on a protein substrate via neutravidin-biotin interactions,in a quantitatively and spatially controllable manner. Such Wnt3a gradientis bioactive as it triggers a dose- and time-dependent upregulation of β-cateninand cell proliferation, in model mouse L cells. Most importantly, human hairdermal papilla cells (HHDPCs) cultured in a cell niche with Wnt3a gradientproliferate in a Wnt3a-gradient-dependent manner and exhibit a Wnt3a-gradient-dependent hair-inductive phenotype including Wnt/β-catenin signalingactivation and alkaline phosphatase (ALP) expression. This work contributesto the future development of a hair follicle inductivity biochip for screening ofdrugs and therapeutics on hair regeneration.

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《Advanced functional materials》 |2023年第33期|2301941.1-2301941.17|共17页
作者
Xinna Wang; Wanjing Ou; Di FengBo GaoBarbara Pui Chan;
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作者单位

Tissue Engineering LaboratoryDepartment of Mechanical EngineeringThe University of Hong KongPokfulam Road, Hong Kong, Hong Kong SAR, P. R. China;

School of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongShatin, N.T., Hong Kong, Hong Kong SAR, P. R. China,School of Biomedical SciencesLi Ka Shing Faculty of MedicineThe University of Hong KongPokfulam Road, Hong Kong SAR, P. R. China;

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正文语种英语
中图分类无线电电子学、电信技术;工程材料学;
关键词
growth factor immobilization; human hair dermal papilla cells; multiphoton microfabrication; protein micropatterns; Wnt/β-catenin signaling;

A Bio-Functional Wnt3a Gradient Microarray for Cell Niche Studies

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