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首页> 外文期刊>Applied Microbiology and Biotechnology >Real-time metabolic heat-based specific growth rate soft sensor for monitoring and control of high molecular weight hyaluronic acid production by Streptococcus zooepidemicus
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Real-time metabolic heat-based specific growth rate soft sensor for monitoring and control of high molecular weight hyaluronic acid production by Streptococcus zooepidemicus

机译：Real-time metabolic heat-based specific growth rate soft sensor for monitoring and control of high molecular weight hyaluronic acid production by Streptococcus zooepidemicus

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摘要

This present investigation addressing the metabolic bottleneck in synthesis of high MW HA by Streptococcus zooepidemicus and illustrates the application of calorimetric fed-batch control of mu at a narrower range. Feedforward (FF) and feedback (FB) control was devised to improve the molecular weight (MW) of HA production by S. zooepidemicus. Metabolic heat measurements (Fermentation calorimetry) were modeled to decipher real-time specific growth rate, mu(est) was looped into the PID circuit, envisaged to control mu(SP) to their desired setpoint values 0.05 h(-1), 0.1 h(-1), and 0.15 h(-1) respectively. Similarly, a predetermined exponential feed rate irrespective of real-time mu was carried out in FF strategy. The developed FB strategy established a robust control capable of maintaining the specific growth rate (mu) close to the mu(SP) value with a minimal tracking error. Exponential feed rate carried out with a lowest mu(SP) of 0.05 h(-1) showed an improved MW of HA to 2.98 MDa and 2.94 MDa for the FF and FB-based control strategies respectively. An optimal HA titer of 4.73 g/L was achieved in FF control strategy at mu(SP) = 0.15h(-1). Superior control of mu at low mu(SP) value was observed to influence HA polymerization positively by yielding an improved MW and desired polydispersity index (PDI) of HA. PID control offers advantage over conventional fed-batch method to synthesize HA at an improved MW. Calorimetric signal-based mu control by PID negates adverse effects due to the secretion of other end products albeit maintaining regular metabolic activities.

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