Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids

Koudan Elizaveta V; Zorina Alla I; Levin Aleksandr A.Pereira Frederico D. A. S.Petrov Stanislav VKarshieva Saida ShKasyanov Vladimir A.Manturova Natalya E.Ustyugov Andrey YuPotekaev Nikolay N.Parfenov Vladislav A.Karalkin Pavel A.Khesuani Yusef D.Bulanova Elena A.Kopnin Pavel B.Isaev Artur A.Mironov Vladimir A.Zorin Vadim L.

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Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids

机译：Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids

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In situ 3D bioprinting is a new emerging therapeutic modality for treating human skin diseases. The tissue spheroids have been previously suggested as a powerful tool in rapidly expanding bioprinting technology. It has been demonstrated that the regenerative potential of human dermal fibroblasts could be quantitatively evaluated in 2D cell culture and confirmed after implantation in vivo. However, the development of unbiassed quantitative criteria of the regenerative potential of 3D tissue spheroids in vitro before their in situ bioprinting remains to be investigated. Here it has been demonstrated for the first time that specific correlations exist between the regenerative potential of human dermal fibroblasts cultured in vitro as 2D cell monolayer with biological properties of 3D tissue spheroids fabricated from these fibroblasts. In vitro assessment of biological properties included diameter, spreading and fusion kinetics, and biomechanical properties of 3D tissue spheroids. This comprehensive characterization could be used to predict tissue spheroids' regenerative potential in vivo.

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《Cell and Tissue Research》 |2022年第3期|453-464|共12页
作者
Koudan Elizaveta V; Zorina Alla I; Levin Aleksandr A.Pereira Frederico D. A. S.Petrov Stanislav VKarshieva Saida ShKasyanov Vladimir A.Manturova Natalya E.Ustyugov Andrey YuPotekaev Nikolay N.Parfenov Vladislav A.Karalkin Pavel A.Khesuani Yusef D.Bulanova Elena A.Kopnin Pavel B.Isaev Artur A.Mironov Vladimir A.Zorin Vadim L.;
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作者单位

NN Priorov Natl Med Res Ctr Traumatol & Orthoped;

Human Stem Cells Inst;

National University of Science & Technology (MISIS),Natl Univ Sci & Technol MISISMinistry of Health of the Russian Federation,Minist Hlth Russian FederatRiga Stradins UnivPirogov Russian Natl Res Med Univ RNRMUSechenov Univ,Minist Hlth Russian FederatChem Divers Res Inst;

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正文语种英语
中图分类细胞生物学;
关键词
Dermal fibroblasts; Clonogenicity; Proliferation; Regenerative potential; Tissue spheroids; DIFFERENTIAL ADHESION; BONE-MARROW; CELLS; COLONIES; SURFACE;

Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids

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