Synthesis of Crosslinked 2'-OMe RNA Duplexes Using 2-Amino-6-Vinylpurine and Their Application for Effective Inhibition of miRNA Function

Ahmed Mostafa Abdelhady; Yu Hirano; Kazumitsu OnizukaHidenori OkamuraYasuo Komatsuand Fumi Nagatsugi

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Synthesis of Crosslinked 2'-OMe RNA Duplexes Using 2-Amino-6-Vinylpurine and Their Application for Effective Inhibition of miRNA Function

机译：Synthesis of Crosslinked 2'-OMe RNA Duplexes Using 2-Amino-6-Vinylpurine and Their Application for Effective Inhibition of miRNA Function

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Crosslinking reactions to nucleic acids are an effective way to prepare stable complexes formed by covalent bonding. We demonstrated that fully 2'-O-methylated (2'-OMe) RNAs having a 2-amino-6-vinylpurine (AVP) exhibited an efficient crosslinking to uracil in the target RNA. Recently, we reported the preparation of crosslinked 2'-OMe RNA duplexes using AVP and the anti-miRNA oligonucleotides (AMOs) containing crosslinked duplexes at the terminal positions. These AMOs exhibited efficient microRNA (miRNA) inhibition at very low concentrations. In this article, we describe the chemical synthesis of 2'-OMe oligonucleotides containing AVP and preparation of the AMOs bearing crosslinked 2'-OMe RNA duplexes using AVP. In addition, we describe in detail the miRNA inhibition assay using these AMOs.

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《Current Protocols in Nucleic Acid Chemistry》 |2022年第3期|e386-1-e386-17|共17页
作者
Ahmed Mostafa Abdelhady; Yu Hirano; Kazumitsu OnizukaHidenori OkamuraYasuo Komatsuand Fumi Nagatsugi;
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作者单位

Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Miyagi, Japan;

Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Toyohira-ku, Sapporo, Hokkaido, Japan;

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正文语种英语
中图分类生物化学;
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anti-miRNA; crosslinked duplex; nucleoside; oligonucleotide;

Synthesis of Crosslinked 2'-OMe RNA Duplexes Using 2-Amino-6-Vinylpurine and Their Application for Effective Inhibition of miRNA Function

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