The In Vitro Study of Anti-leishmanial Effect of Naja naja oxiana Snake Venom on Leishmania major  Bentham Science

Nastaran Fallahi; Delavar Shahbazzadeh; Fatemeh MalekiMarjaneh AghdasiFatemeh TabatabaieKhadijeh Khanaliha

首页> 外文期刊>Infectious disorders drug targets >The In Vitro Study of Anti-leishmanial Effect of Naja naja oxiana Snake Venom on Leishmania major Bentham Science

【24h】

The In Vitro Study of Anti-leishmanial Effect of Naja naja oxiana Snake Venom on Leishmania major Bentham Science

机译：The In Vitro Study of Anti-leishmanial Effect of Naja naja oxiana Snake Venom on Leishmania major Bentham Science

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Background: Although a majority of patients with cutaneous leishmaniasis (CL) arehealed with Glucantime chemotherapy, the increased drug resistance rate following its application isa concern. In this study, Naja naja oxiana crude venom of cobra snakes was used for the first timeas an assembled combination of bioactive pharmaceutical components on Leishmania major (L.major) standard strain.Objective: To evaluate the efficacy of the Naja naja oxiana crude venom of Iranian cobra snakes onLeishmania major standard strain in vitro.Methods: Five concentrations (1.25, 2.5, 5, 10 and 20 μg/mL) of venom were added to Leishmaniamajor cultures at 24, 48 and 72 h. The viability of the parasites and venom toxicity were assessed byMTT test. The apoptosis was determined by flow cytometry, while IC_(50) was determined by countingparasites compared to that of glucantime. Each test was conducted in triplicate.Results: After the exposure of the venom for 72 h, IC_(50) value was 0.36 μg/mL and 14.12 μg/mL forpromastigotes and amastigotes, respectively. MTT valuation clarified 1.01% promastigotes viability.Furthermore, the results indicated that the venom of Naja naja oxiana induced early and lateapoptosis in parasites.Conclusions: The venom of Naja naja oxiana revealed remarkable anti-leishmanial effects as anovel anti-parasitic alternative. Thus, the bioactive and effective fractions of this venom may beconsidered as anti-leishmanial candidates in future studies.

著录项

来源
《Infectious disorders drug targets》 |2020年第6期|913-919|共7页
作者
Nastaran Fallahi; Delavar Shahbazzadeh; Fatemeh MalekiMarjaneh AghdasiFatemeh TabatabaieKhadijeh Khanaliha;
展开▼
作者单位

Department of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences;

Venom and Biotherapeutics Molecules Lab,Medical Biotechnology Department, Biotechnology Research;

Department of Parasitology, Faculty of Para Medical Sciences, Iran University of Medical SciencesDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat Modares UniversityResearch Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类药学;
关键词
Iranian cobra; Naja naja oxiana; Leishmania major; MTT; apoptosis; IC50;

The In Vitro Study of Anti-leishmanial Effect of Naja naja oxiana Snake Venom on Leishmania major Bentham Science

摘要

著录项

相关主题

期刊订阅