Bioinformatic analysis and relative expression of hyd and fdx during H2 production in microalgae

Yahaira de Jesús Tamayo‐Ordoñez; Benjamín Abraham Ayil‐Gutiérrez; Ileana Mayela Maria Moreno‐DavilaFrancisco Alberto Tamayo‐OrdoñezAtl Victor Córdova‐QuirozWilberth Alfredo Poot‐PootSiprian Damas‐DamasHernán de Jesús Villanueva‐AlonzoMaria Concepción Tamayo‐Ordoñez

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Bioinformatic analysis and relative expression of hyd and fdx during H2 production in microalgae

机译：Bioinformatic analysis and relative expression of hyd and fdx during H2 production in microalgae

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SUMMARY Microalgae have been positioned as excellent models for producing new sources of energy (biofuels and biohydrogen). Some investigations in these biological models have been directed to know if the enzymes ferredoxin (FDX) and hydrogenase (HYD) are involved in the algae producing different concentrations of molecular hydrogen (H2). To date, little is known about the concomitant transcriptional regulation of both enzymes during H2 evolution in algae. In this research, we evaluated the relative expression of hdy and fdx genes during the evolution of H2 in three microalgae (Chlorella vulgaris, Scenedesmus obliquus, and Chlamydomonas reinhardtii) in N‐deprived anaerobic cultures in the presence of Fe, and 12:24 and 24:24 h dark:light cycles. We also detected structural differences in the enzymes. The 3D modeling indicated that the 3D structure of HYD and FDX are conserved in most algal genera, and the results of our grouping according to the aa characteristics of the proteins showed two grouping trends: One, according to the algae's phylogenetic classification, and another one according to the species‐specific enzyme's characteristics, and the grouping could perhaps be more influenced by the algae's ability to produce H2. The three microalgae species reached maximum H2 accumulation values in 24h:24 h dark:light conditions in Fe‐supplemented media (4.2 ± 0.12 mL L−1 in C. vulgaris, 3.9 ± 0.10 mL L−1 in S. obliquus, and 4.5 ± 0.10 mL L−1 in C. reinhardtii), and the highest global relative expression of hyd and fdx genes was reached during the first hour of exposure to light, which suggests concomitant expression of both enzymes at the beginning of H2 production. The behavior of the expression of the hyd and fdx genes in these algal species proved to be similar between species. A better understanding of the concomitant regulation of both enzymes could lay the groundwork for the future use of both enzymes to improve H2 yields in microalgae.

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《Phycological Research》 |2023年第1期|37-55|共19页
作者
Yahaira de Jesús Tamayo‐Ordoñez; Benjamín Abraham Ayil‐Gutiérrez; Ileana Mayela Maria Moreno‐DavilaFrancisco Alberto Tamayo‐OrdoñezAtl Victor Córdova‐QuirozWilberth Alfredo Poot‐PootSiprian Damas‐DamasHernán de Jesús Villanueva‐AlonzoMaria Concepción Tamayo‐Ordoñez;
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Universidad Autónoma de Coahuila;

Instituto Politécnico Nacional, Biotecnología Vegetal;

Universidad Autónoma del CarmenCentro Universitario Victoria “Adolfo López Mateos”CONACYT‐Centro de Investigaciones Regionales Dr. Hideyo Noguchi (CIR)‐Universidad Autónoma de Yucatán (UADY). Laboratorio de Biología Celular;

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正文语种英语
中图分类植物学;
关键词
ferredoxin gene; H2 production; hydrogenase gene; microalgae; mutation;

Bioinformatic analysis and relative expression of hyd and fdx during H2 production in microalgae

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