Vibrio cholerae adapts to sessile and motile lifestyles by cyclic di-GMP regulation of cell shape

Fernandez Nicolas L.; Hsueh Brian Y.; Nhu Nguyen T. Q.Franklin Joshua L.Dufour Yann S.Waters Christopher M.

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Vibrio cholerae adapts to sessile and motile lifestyles by cyclic di-GMP regulation of cell shape

机译：Vibrio cholerae adapts to sessile and motile lifestyles by cyclic di-GMP regulation of cell shape

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The cell morphology of rod-shaped bacteria is determined by the rigid net of peptidoglycan forming the cell wall. Alterations to the rod shape, such as the curved rod, occur through manipu-lating the process of cell wall synthesis. The human pathogen Vibrio cholerae typically exists as a curved rod, but straight rods have been observed under certain conditions. While this appears to be a regulated process, the regulatory pathways controlling cell shape transitions in V. cholerae and the benefits of switching between rod and curved shape have not been determined. We demonstrate that cell shape in V. cholerae is regulated by the bacterial second messenger cyclic dimeric guanosine monophosphate (c-di-GMP) by post transcriptionally repressing expression of crvA, a gene encoding an intermediate filament-like protein necessary for curvature formation in V. cholerae. This regulation is mediated by the transcriptional cascade that also induces production of biofilm matrix components, indicating that cell shape is coregulated with V. cholerae's induction of sessility. During microcolony formation, wild-type V. cholerae cells tended to exist as straight rods, while genetically engineering cells to maintain high curvature reduced microcolony formation and biofilm density. Conversely, straight V. cholerae mutants have reduced swimming speed when using flagellar motility in liquid. Our results demonstrate regulation of cell shape in bacteria is a mechanism to increase fitness in planktonic and biofilm lifestyles.

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《Proceedings of the National Academy of Sciences of the United States of America.》 |2020年第46期|29046-29054|共9页
作者
Fernandez Nicolas L.; Hsueh Brian Y.; Nhu Nguyen T. Q.Franklin Joshua L.Dufour Yann S.Waters Christopher M.;
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作者单位

Dept Microbiol & Mol Genet,Michigan State Univ;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
cell shape; crvA; cyclic di-GMP; biofilm; motility; BIOFILM FORMATION; MORPHOLOGY; VPST; GENES; EXOPOLYSACCHARIDE; ARCHITECTURE; MODULATION; EXPRESSION; RUGOSITY;

Vibrio cholerae adapts to sessile and motile lifestyles by cyclic di-GMP regulation of cell shape

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