Prevalence and Molecular Genotyping of Cryptosporidium Spp. in Diarrheic Patients from Bandar Abbas City, Southern Iran

Majid Najafi-Asl; Habibollah Faraji; Saeed Hosseini TeshniziKhojasteh Sharifi-Sarasiabi

首页> 外文期刊>Jundishapur Journal of Microbiology >Prevalence and Molecular Genotyping of Cryptosporidium Spp. in Diarrheic Patients from Bandar Abbas City, Southern Iran

【24h】

Prevalence and Molecular Genotyping of Cryptosporidium Spp. in Diarrheic Patients from Bandar Abbas City, Southern Iran

机译：Prevalence and Molecular Genotyping of Cryptosporidium Spp. in Diarrheic Patients from Bandar Abbas City, Southern Iran

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Background: Cryptosporidium species are recognized as one of the most important gastrointestinal pathogens of humans and livestock. Objectives: This study aimed to determine the prevalence and sub-genotypes of Cryptosporidium spp. among diarrheic patients in Bandar Abbas City, Iran. Methods: Diarrheic fecal samples were collected from 170 patients in three hospitals of Bandar Abbas, Iran, from October 2018 to May 2019. Initial parasitological identification of Cryptosporidium spp. was performed by modified Ziehl-Neelsen (ZN) staining. For molecular analysis, the positive specimens and the suspected ones of Cryptosporidium spp. were evaluated by sequence analysis of the 60-kDa glycoprotein gene (gp60). The collected data were analyzed using SPSS software and the relationship between the variables and the presence of Cryptosporidium spp. assessed by the chi-square test. To assess the degree of agreement between PCR and ZN staining, Cohen’s kappa-index was applied. Results: Of the 170 diarrheic patients, 98 (57.6%) were male, and 72 (42.4%) were female. Prevalence of Cryptosporidium spp. by parasitological examination was 1.8% (3/170). However, using PCR, Cryptosporidium spp. was detected in 12% (6/50) of the positive microscopically samples (3 samples) and 47 suspected specimens. Sequence analysis of the gp60 gene showed that all of the positive isolates were Cryptosporidium parvum in which all subtypes belonged to allele family IId. Two distinct nucleotide sequences obtained from this study were deposited in GenBank under the accession numbers MN820453 and MN820454. Conclusions: The predominance of C. parvum (subtype family IId) in this study emphasizes the importance of zoonotic Cryptosporidium transmission in Bandar Abbas, Southern Iran.

著录项

来源
《Jundishapur Journal of Microbiology》 |2020年第6期|1-7|共7页
作者
Majid Najafi-Asl; Habibollah Faraji; Saeed Hosseini TeshniziKhojasteh Sharifi-Sarasiabi;
展开▼
作者单位

Department of Parasitology, Faculty of Medicine, Hormozgan University of Medical Sciences;

Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences;

Department of Biostatistics, Faculty of Nursing and Midwifery, Hormozgan University of Medical SciencesInfectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类
关键词
Genotypes; Subtypes; Cryptosporidium; gp60 gene; Diarrhea; Iran;

Prevalence and Molecular Genotyping of Cryptosporidium Spp. in Diarrheic Patients from Bandar Abbas City, Southern Iran

摘要

著录项

相关主题

期刊订阅