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首页> 外文期刊>Journal of insect biotechnology and sericology >DNA sequence analysis of a chromosomal aberration in irradiation induced burnt (Bu) mutant of Bombyx mori
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DNA sequence analysis of a chromosomal aberration in irradiation induced burnt (Bu) mutant of Bombyx mori

机译：辐照诱导的Bombyx mori烧伤（Bu）突变体染色体的DNA序列分析

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© 2022, Japanese Society of Sericultural Sciences. All rights reserved.The radiation-induced mutations burnt (Bu) and non-preference A4 (Np-A4) map to chromosome 11 in Bom-byx mori. Bu causes irregularly shaped fifth to eighth larval segments, whereas Np-A4 causes one of the po-lyphagous phenotypes, i.e., feeding on non-mulberry leaves. Homozygotes and trans heterozygotes of these mutations die during embryogenesis, suggesting that the lethality associated with Bu and Np-A4 is allelic. Genetic studies of the Bu and Np-A4 mutation would provide a better understanding of the embryogenesis, mor-phogenesis, and feeding behavior of B. mori. Here we conducted a molecular analysis of the Bu locus. In our crossing experiments, larvae carrying both the Bu and Np-A4 mutations appeared, although they were hypoth-esized to die during embryogenesis. A detailed analysis of the survivors resulted in the identification of a breakage event on chromosome 11. Moreover, it was revealed that the survivors exhibited partial trisomy. Using DNA sequence analysis, the breakpoint in chromosome 11 was narrowed to an intron of Bmzfh3. Embryonic lethality associated with the breakage of the chromosome 11 is discussed.
机译：© 2022年，日本蚕桑学会。保留所有权利。辐射诱导的烧伤突变 （Bu） 和非偏好 A4 突变 （Np-A4） 映射到 Bom-byx mori 的 11 号染色体。Bu 引起形状不规则的第 5 至 8 幼虫节段，而 Np-A4 引起 po-lyphagous 表型之一，即以非桑叶为食。这些突变的纯合子和反式杂合子在胚胎发生过程中死亡，表明与 Bu 和 Np-A4 相关的致死性是等位基因。对 Bu 和 Np-A4 突变的遗传研究有助于更好地了解 B. mori 的胚胎发生、形态发生和摄食行为。在这里，我们对 Bu 基因座进行了分子分析。在我们的杂交实验中，出现了携带 Bu 和 Np-A4 突变的幼虫，尽管它们在胚胎发生过程中被假设死亡。对幸存者的详细分析导致确定了 11 号染色体上的断裂事件。此外，据透露，幸存者表现出部分三体性。使用 DNA 序列分析，将 11 号染色体中的断点缩小到 Bmzfh3 的内含子。 讨论了与11号染色体断裂相关的胚胎致死率。

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    Fujii T.; Kubo M.; Kuwazaki S.Yamamoto K.Ohnuma A.Banno Y.Shimada T.;

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    Laboratory of Creative Science for Insect Industries Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences;

    Department of Agricultural and Environmental Biology Graduate School of Agricultural and Life Sciences The University of Tokyo;

    National Institute of Agrobiological ScienceInstitute of Sericulture and Silk Science The Dainippon Silk FoundationLaboratory of Silkworm Genetic Resources Institute of Genetic Resources Kyushu University Graduate School of Bioresources and Bioenvironmental ScienceDepartment of Life Science Faculty of Science/Graduate School of Science Gakushuin University;

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  • 正文语种 英语
  • 中图分类 蚕桑;
  • 关键词

    chromosomal aberration; deletion; DNA sequencing; lethal mutant; polyphagous mutant;

    机译：染色体畸变;删除;DNA测序;致命突变体;多噬突变体;

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