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首页> 外文期刊>Journal of insect biotechnology and sericology >Simple method of ovary transplantation for transgenic silkworm, Bombyx mori, using paraffin coated paper
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Simple method of ovary transplantation for transgenic silkworm, Bombyx mori, using paraffin coated paper

机译：使用石蜡涂层纸对转基因蚕 Bombyx mori 进行卵巢移植的简单方法

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© 2021, Japanese Society of Sericultural Sciences. All rights reserved.We developed a new incision sealing method using a paraffin coated paper (PP) instead of melted paraffin (MP) as in the previous method, to improve the ovary transplantation in silkworm, Bombyx mori. Thus, we suc-ceeded in simplifying the incision sealing without reducing the survival rate of recipient larvae. The survival rate of MP, PP, and no sealing (control) was 65.0 (SE 12.6), 75.6 (SE 13.1), and 50.6 (SE 14.0), respectively. Although, among the above three ways, there was no statistical significance, the PP treatment was easier than the MP one. Next, to effectively use cryopreserved resources, we compared three transplantation methods using normal silkworms as recipients and transgenic silkworms as donors: (A) both recipient ovaries were trans-planted, (B) only one of the ovaries was transplanted, and the other recipient ovary was removed, and (C) only one of the ovaries was operated like in (B), but the other was still kept in the recipient body. Consequently, the method (C), only one-ovary transplantation, was as high as, or more than, other methods in the effectiveness of the reproduction from the frozen-thawed ovary. Our results suggest that the two methods using PP sealing and one ovary transplantation could be convenient and effective in cryopreservation technology using ovary of silk-worm.
机译：© 2021年，日本蚕桑学会。保留所有权利。我们开发了一种新的切口封闭方法，使用石蜡涂层纸（PP）代替以前的熔融石蜡（MP），以改善家蚕的卵巢移植。因此，我们成功地简化了切口密封，而不会降低受体幼虫的存活率。MP、PP和无密封（对照）的存活率分别为65.0%（SE 12.6）、75.6%（SE 13.1）和50.6%（SE 14.0）。虽然在上述三种方法中，没有统计学意义，但PP处理比MP处理更容易。接下来，为了有效利用冷冻保存的资源，我们比较了三种以正常蚕为受者、转基因蚕为供体的移植方法：（A）两个受体卵巢均被转植，（B）仅移植一个卵巢，切除另一个受体卵巢，（C）仅像（B）中一样对一个卵巢进行手术，但另一个仍保留在受体体内。因此，仅单卵巢移植的方法（C）在冷冻解冻卵巢繁殖的有效性方面与其他方法一样高或更高。结果表明，PP封闭和1种卵巢移植两种方法在蚕卵巢冷冻保存技术中具有方便有效的效果。

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