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首页> 外文期刊>Marine ecology >Metabarcoding reveals marked seasonality and a distinctive winter assemblage of dinoflagellates at a coastal LTER site in the Gulf of Naples
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Metabarcoding reveals marked seasonality and a distinctive winter assemblage of dinoflagellates at a coastal LTER site in the Gulf of Naples

机译：Metabarcoding reveals marked seasonality and a distinctive winter assemblage of dinoflagellates at a coastal LTER site in the Gulf of Naples

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Dinoflagellates constitute an abundant and diversified component of marine plankton,mostly associated with stratified conditions typical of late spring through autumnin temperate regions. Yet, difficulties with the identification of many species limitthe knowledge of their composition and seasonal succession. Here we use a 3-yearV4-18SrDNA metabarcoding dataset (4,366,007 reads, 4650 Amplicon SequenceVariants, ASVs) collected on 48 dates at the Long-TermEcological Research siteMareChiara (LTER–MC)to explore the diversity and temporal distribution of dinoflagellatesin the Gulf of Naples (Mediterranean Sea). A large fraction of dinoflagellateASVs (55%, 68% of the reads) were assigned to 187 species in 85 genera, while 33%(23% of the reads) were attributed to undetermined Syndiniales, and the remainderto unidentified groups. A total of 147 ASVs were assigned at 100% similarity to 135reference sequences, corresponding to 116 species, 46 of which were putative newrecords for the Gulf of Naples. Cluster analysis conducted on a normalized datasetof 1199 ASVs identified four sample clusters that corresponded largely to the spring,summer, autumn and winter seasons, respectively, each including samples from all3 years. Syndiniales were particularly abundant (43.1%) in the winter cluster, whereasGyrodinium reached higher percentages in the summer and autumn ones (38.6%and 57.8%), Gymnodiniales sensu stricto were more abundant in spring (11.5%) andGonyaulacales in summer (13.5%). Almost half of the ASVs analysed (561) were significantlyassociated with one of the four seasonal clusters (p < .05). The winter groupof ASVs was particularly rich (348 ASVs) and mainly consisted of naked and parasitictaxa, which are impossible to identify in routine phytoplankton observations in lightmicroscopy. Despite biases mainly ascribed to both the incompleteness of the referencedataset and the limited resolution of the marker gene, the metabarcoding approachhas provided new insights into the ecology and distribution of this importantcomponent of the plankton, highlighting its marked seasonality along with the existenceof a diversified and previously overlooked dinoflagellate community in winter.

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