Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes

The Mon La; Hiromi Tachibana; Shun‐Ai LiTadashi AbeSayaka SeirikiHikaru NagaokaEizo TakashimaTetsuya TakedaDaisuke OgawaShin‐ichi MakinoKatsuhiko AsanumaMasami WatanabeXuefei TianShuta IshibeAyuko SakaneTakuya SasakiJun WadaKohji TakeiHiroshi Yamada

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Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes

机译：Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes

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Abstract Dynamin 1 is a neuronal endocytic protein that participates in vesicle formation by scission of invaginated membranes. Dynamin 1 is also expressed in the kidney; however, its physiological significance to this organ remains unknown. Here, we show that dynamin 1 is crucial for microtubule organization and stabilization in glomerular podocytes. By immunofluorescence and immunoelectron microscopy, dynamin 1 was concentrated at microtubules at primary processes in rat podocytes. By immunofluorescence of differentiated mouse podocytes (MPCs), dynamin 1 was often colocalized with microtubule bundles, which radially arranged toward periphery of expanded podocyte. In dynamin 1‐depleted MPCs by RNAi, α‐tubulin showed a dispersed linear filament‐like localization, and microtubule bundles were rarely observed. Furthermore, dynamin 1 depletion resulted in the formation of discontinuous, short acetylated α‐tubulin fragments, and the decrease of microtubule‐rich protrusions. Dynamins 1 and 2 double‐knockout podocytes showed dispersed acetylated α‐tubulin and rare protrusions. In vitro, dynamin 1 polymerized around microtubules and cross‐linked them into bundles, and increased their resistance to the disassembly‐inducing reagents Ca2+ and podophyllotoxin. In addition, overexpression and depletion of dynamin 1 in MPCs increased and decreased the nocodazole resistance of microtubules, respectively. These results suggest that dynamin 1 supports the microtubule bundle formation and participates in the stabilization of microtubules.

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《The FASEB Journal》 |2020年第12期|16449-16463|共15页
作者
The Mon La; Hiromi Tachibana; Shun‐Ai LiTadashi AbeSayaka SeirikiHikaru NagaokaEizo TakashimaTetsuya TakedaDaisuke OgawaShin‐ichi MakinoKatsuhiko AsanumaMasami WatanabeXuefei TianShuta IshibeAyuko SakaneTakuya SasakiJun WadaKohji TakeiHiroshi Yamada;
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作者单位

Department of Neuroscience,Okayama University;

Department of Nephrology, Rheumatology, Endocrinology and Metabolism,Okayama University;

Center for Innovative Clinical Medicine,Okayama University HospitalDivision of Malaria Research,Ehime UniversityDepartment of Nephrology,Chiba UniversityDepartment of Internal Medicine,Yale University School of MedicineDepartment of Biochemistry,Tokushima University Graduate School of Medical Sciences;

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正文语种英语
中图分类生物化学;
关键词
dynamin; microtubules; podocyte; primary process;

Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes

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