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首页> 外文期刊>Bioprocess and biosystems engineering >Production of lactobionic acid at high salt concentrations by Acinetobacter halotolerans isolated from seaside soil
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Production of lactobionic acid at high salt concentrations by Acinetobacter halotolerans isolated from seaside soil

机译：Production of lactobionic acid at high salt concentrations by Acinetobacter halotolerans isolated from seaside soil

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Abstract A lactobionic acid (LBA)-producing bacterium isolated from seaside soils was identified as Acinetobacter halotolerans and designated as strain KRICT-1. We determined whether KRICT-1 can produce LBA at high salt concentrations. The KRICT-1 strain grew on a nutrient broth (NB) agar plate with up to 7.0% NaCl, indicating high NaCl tolerance, and 30?°C was the optimum growth temperature for LBA production. We produced LBA using the KRICT-1 strain in NB medium containing various concentrations of NaCl. While Pseudomonas taetrolens, an efficient LBA-producing bacterium, could produce LBA with up to 5.5% NaCl, the KRICT-1 strain could produce LBA at up to 7.0% NaCl and produced more LBA than P. taetrolens with over 5.5% NaCl. We produced LBA using NB medium containing 7.0% NaCl by batch fermentation of the KRICT-1 strain in a 5 L fermenter. The LBA production titer and productivity of the KRICT-1 strain were 32.1?g/L and 0.22?g/L/h, respectively, which were approximately 1.35- and 1.38-fold higher than those (23.7?g/L and 0.16?g/L/h) obtained from flask culture. Additionally, quinoprotein glucose dehydrogenase is an LBA-producing enzyme in A. halotolerans. We demonstrated that the A. halotolerans KRICT-1 strain is appropriate for LBA production at high salt concentrations.

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