Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds

Katrina L. Wilson; Sasha Cai Lesher Perez; Moawiah M. NaffaaSean H. KellyTatiana Segura

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Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds

机译：Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds

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Microporous annealed particle (MAP) scaffolds are generated from assembledhydrogel microparticles (microgels). It has been previously demonstratedthat MAP scaffold are porous, biocompatible, and recruit neural progenitorcells (NPCs) to the stroke cavity after injection into the stroke core. Here,the goal is to study NPC fate inside MAP scaffolds in vitro. To create plainmicrogels that can later be converted to contain different types of bioactivities,the inverse electron-demand Diels-Alder reaction between tetrazine andnorbornene is utilized, which allows the post-modification of plain microgelsstoichiometrically. As a result of adhesive peptide attachment, NPC spreadingleads to contractile force generation which can be recorded by trackingmicrogel displacement. Alternatively, non-adhesive peptide integration resultsin neurosphere formation that grows within the void space of MAP scaffolds.Although the formed neurospheres do not impose a contractile forceon the scaffolds, they are seen to continuously transverse the scaffolds. It isconcluded that MAP scaffolds can be engineered to either promote neurogenesisor enhance stemness depending on the chosen post-modificationsof the microgels, which can be key in modulating their phenotypes in variousapplications in vivo.

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《Advanced Materials》 |2022年第33期|2201921.1-2201921.12|共12页
作者
Katrina L. Wilson; Sasha Cai Lesher Perez; Moawiah M. NaffaaSean H. KellyTatiana Segura;
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Department of Biomedical EngineeringDuke UniversityDurham, NC 27708-0281, USA;

Department of Chemical EngineeringUniversity of MichiganNorth Campus Research ComplexBuilding 28, 2800 Plymouth Rd, Ann Arbor, MI 48109-2800, USA;

Department of Cell BiologyDuke University School of MedicineDurham, NC 27710, USA, Department of Psychology and NeuroscienceDuke UniversityDurham, NC 27708, USADepartment of Biomedical EngineeringDuke UniversityDurham, NC 27708-0281, USA, Department of NeurologyDuke UniversityDurham, NC 27708-0281, USA, Department of DermatologyDuke UniversityDurham, NC 27708-0281, USA;

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hydrogels; microgel/microparticles; microporous annealed particles; neural progenitor cells; neural stem cells; norbornene; tetrazine;

Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds

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